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Publication Expression of the Recombinant ORF 2 Protein of Porcine Circovirus Ty...  

Update Date: [2013-11-14]

NO.: 42
 
Reportno. AHRI report No.42
Topic Expression of the Recombinant ORF 2 Protein of Porcine Circovirus Type 2 in Eukaryotic System
Department 1Animal Health Research Institute, Council of Agriculture, Executive Yuan 
2Graduate Institute of Veterinary Medicine , National Taiwan University
Author

Chun Wang*1.2, Jui-Yu Chiu1.2, Tien-Shine Huang1, Ming-Hwa Jong1, Parn-Hwa Zhao1 and Ing -Cherng Guo

Summary
Porcine circovirus type 2 represents the causative agent of a new porcine disease which spread all over the world during the past several years. In this study, viral DNA of a Taiwanese PCV2 strain was extracted and its open reading frame 2 (ORF2) gene was amplified by PCR. The PCR product was purified and then cloned into the pCI-neo vectors. The recombinant vector was transfected into PK-15 cells. The recombinant protein was expressed successfully in PK-15 cells, which could be detected by using indirect immunofluorescence assay and Western blotting analysis. 

Keyword

Porcine circovirus type 2; Indirect immunofluorescense assay; SDS-PAGE; Western blotting