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Publication Prussian Blue Test- Spot Formation Method for Rapid Detection of Cya...  

Update Date: [2013-11-14]

NO.: 42
Reportno. AHRI report No.42
Topic Prussian Blue Test- Spot Formation Method for Rapid Detection of Cyanide-Poisoned Specimens
Department 1 Animal Health Research Institute, Council of Agriculture, Executive Yuan 
2 Graduate Department of Animal Science, Chinese Culture University , Taipei 
3 Department of Computer Science, Tsing Hua University , Hsinchu 
4 Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Taipei
Author W. H. Lin*1, C. F. Chang2, C.C. Chen3, L. J. Weng4, H.T. Sung4, P. H. Chao1
This study aimed to establish rapid detection techniques for cyanide intoxication cases by using the prussian blue test, a spot formation method (PBT-SFM). In practice, cyanide-containing vapor was passed through ferrous filter paper via vacuum system to form ferriferrocyanide spots in situ after being released from specimen with sulfuric acid in an improved reaction chamber.. With subsequent de-coloring process by hydrochloride, visible blue spots were measured by digital image processing and calibration curve regression. Preliminary experimental result at 5, 10, 25, and 50 μg standard doses demonstrated that the minimal detection concentration of standard solution was 150 μg/L or 2.3 μM based on the calibration curve at regression coefficient of 0.9727. Practical detection on collected clinical specimens of stomach content and visceral organs showed negative results. The study established a semi-quantitative test that would not require sophisticated equipment or expensive reagents for rapid detecting cyanide in poisoned specimens by clinical laboratory.
Keyword Animal, Poisoning, Cyanide, Prussian blue test, Spot formation method