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Research Information Development and application of recombinant proteins of porc...  

Update Date: [2013-11-01]

Plan Year: 2006
 
    Viral DNA of Taiwanese PCV2 was extracted and the ORF2-encoding region was amplified by PCR.  The PCR product was inserted into the different vectors, and the expressed proteins in eukaryocytes were identified further with anti-PCV2 polyclonal antibody by Western blotting.  Recombinant ORF2 protein will be purified with HisTrap column, and coated on the surface of 96-well ELISA plates.  The antibodies titers of the sera from different farms and age groups will be determined by ELISA.  We have designed different peptide-based antigen for PCV2 effective in swine.  The peptide immunogen have various epitope from the ORF2 capsid protein of PCV2.  The safe chemically-defined product would have considerable advantages for immuno-dot assays to detect antibody titers of serums..  On the other hand, real-time PCR will be applied to detect antigens of PCV2 in serums.