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Publication Rapid and Simultaneous Determination of Water Soluble Vitamins(B1、B2...  

Update Date: [2013-11-11]

NO.: 29
 
Reportno.

AHRI report No.29

Topic

Rapid and Simultaneous Determination of Water Soluble Vitamins(B1、B2、B6、Niacin) in Premixes by High Performance Liquid Chromatography.
 

Department

Taiwan Animal Health Research Institute The Branch Institute of Animal Drugs epa

Author KM. Un )vI.C. Uu5.Y. lin.P. Liu
Summary

Premixes were getreatel dixeDily with a little mobile phase, then added to the volurme. a mixing and filtration, the filtrate was infrim into a high performance liquid chromatography (HPLQ which was equipped with a BECKMAN OCI’YL 4.6X250 mm column and 275 nm detr. The mobile phase was 5mM PICB5(Ijust topH 3.25 withaceticacid) : methanol=71:29. WaterSoluble Vitamins (Niacin, B6, Bi, B2) in Premixes could ke determined simultaneously. The retention times of Niacin 1(I) mcg.ini, Vit. B6 50 mcghni, Vit. Bi 50 mcghnl, Vit. B2 50 mcgknl were 3.28, 4.2Z5.47 and 6,82 minute, reseDtively. Using HPLC, the Water Soluble Vitamins (eg. Niacin, B6, B 1, B2) in premixes can ke determined rapidly and simultaneously and the risk of exposure to toxic organic solvent (eg. BrCN), the time and operation task can ke reducer!.

Keyword

Water soluble vitamins- emix-Simultansousassay High Performance Liquid nt-lw Cnatogxaphy