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Publication THE DEVELOPMENT OF ENZYME CONJUGATION AND ITS TECHNIC IMPROVENT  

Update Date: [2013-11-06]

NO.: 24
 
Reportno.

AHRI report No.24

Topic

THE DEVELOPMENT OF ENZYME CONJUGATION AND ITS TECHNIC IMPROVENT

Department

1.Animal Health Research lirstitue, (Suncil of Agriculture
2.National Taiwi Q&ge Of Nursing

Author T.S. Huangl ; Andrew, C.Y. Fei 1; PH. Mar2
Summary

Rabbit anti-swine IgO antibody was conjugatd with horse racklish roxidase by the gluldehyde arid Nakarie methcds to evalua the effect of the two conjugation methcds.

Result showi the product of the glutklehyde rnethcd could lie further purified by Summary CcmA-sepharose to scearate the free antibcdy and the conjugatl antibody, The product of & Nakane rnehtod could not lie further purified by the ConA-sepharose ltause the sugar of the enzyme was oxygenatl in the a njugationprcxess. The rnolarity ratio of enzymekntibcdy of the glutaldehyole and Nakarie methcds were 1.5 and 0.9 respaDtively.

Keyword

ConA-sepharoseRabbit Nakane rnethcds