Home
 /  Publication  /  Volum 33  /  Publication Investigation of Enrofloxacin and Ofloxacin Residues in Po...
Publication Investigation of Enrofloxacin and Ofloxacin Residues in Poultry tiss...  

Update Date: [2013-11-13]

NO.: 33
 
Reportno.

AHRI report No.33

Topic

Investigation of Enrofloxacin and Ofloxacin Residues in Poultry tissues

 

Department

Taiwan Animal Health Researdi Institute The Branch Institute of Animal Dnigs eparimen

Author Y.Y. Shen9’.S. (JongM.C. Liu$.Y. Un
Summary

A high-erfoxmance liquid chromatographic (HPLQ method for simultaneous determination of the residue of Enxofloxacin (ERFX) and Ofloxacin (OFLX) in poultry tissues was developel. Samples were extracted and purified with 0.O1N Hydrcchloric acid-Acetoniti-ile (7:3 v /v) and SEP-PAK Cl 8, concentrated by nitrogen to dryness, and then analysed by HPLC. The HPLC conditions were established on a Csmccil SC1 8-MS column with pH 3.0 Phosphate buffer /Acetonitile (80:20) as mobile phase  and detected by fluroresence detector Ex 295 nm?Em 443 run, Recovery stdies were performed at 0.05 ppm levels in each of the three tissues, Average recoveries were 82.3-92.6 %. The detection limit of residual drugs was 0(03 ppm in samples.

Total 112 samples of Poultry tissue from grccery market lccaim in seven counties of Taiwan were surveyed by random for the residues of Enroflox&cin and Ofloxacin with the developed method. The result indicatel postive ra of ERFX and OFLX among the samples were 19.6 % (0.095—0.C03 ppm) and 2.7 % (0.09)—0.017 ppm) respectively.

Keyword

Enxofloxacinfloxacin -,Poultry tissue.