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Publication Phylogenetic analysis of classical swine fever virus in Taiwan  

Update Date: [2013-11-13]

NO.: 40

AHRI report No.40


Phylogenetic analysis of classical swine fever virus in Taiwan

Department 1.Depoartment of Hog Cholera, Animal Health Research bsti Council of Agricultuxe Tairei, Taiwan
2.Decartnent of Veterinary Medicine, National Taiwan University, Tai:ei, Taiwan 3 Dertnent of Medical Research, Mackay Memcuial Hctal, Taicei, Taiwan
Author Mau-Jinn Kwarg, Chien-Chih W; Chao-Hsien Lee. Chia-Chen Gang, Shiu-Ru Author YeA and Jei-Fu Su.

Two envelope glycogotein (Ems and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-FCR and srencerl direrztly from 158 soimens collected Iretween 1989 and 2(03 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the Ems region rno’iderl ketter discrimination than the £2 region. One hundred and fifn isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2,la and 2.lb) and 2,Z which were considered to ke likely of the intcwiuced stains, whereas the remaining 43 isola were clustered within subgroup 3.4 and were considered to “ lreofthe endemic strains. The subgroup 2.la viruses were first detecti in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in theearlyyears, ningisolatia&r19%.WehaveoberMadramaticswmohin genotype from subgroup 3.4 to 2.la. The subgroup 2.la isolates are clccsely related to thePderlrornandLaoisolat, whereas 2.lbisolates have a dc6e relationship to the Chinese Guangxi isolates. The phylogenetic tee of 27 CFV sopences based on the complete envelope glycogotein gene (Ems - £2) displayed )ftter resolution than that bised on the complete open reading frame.


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