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Publication A Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Viral Ant...  

Update Date: [2013-11-11]

NO.: 28
 
Reportno.

AHRI report No.28

Topic

A Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Viral Antigens of Infectious Bursal Disease

Department

1.Taiwan Animal Health Research Institute
2.Department of Veterinarv Medicine. National Chung-Hsing University

Author Y.P. LinlFL.H. Lee 2FHappyK. Shieh2
Summary

Monoclonal antibody (mAb) 2E6 against \?2 capsid protein of infectious brusal disease virus (IBDV) or rabbit anti-IBDV antis erurn wasinitially used to capture the IBDV antigens in bursal tissue specimens followed by an enzyme linked intmunosorbent assay (sandwich ELISA) for the detection of ]BD infection. IBDV antigens in 16 from 24 bursal specimens could be detected. The ratio of aborbance value from IBDV-positive and -negative bursal tissues (RN) was 7.5 when the nAb was initially used in sandwich ELISA, while that was 6.9 when rabbit anti-IBDV antiserum was used. It suggests that the sandwich ELISA following the antigen captuure with both kinds of antibody could clearly differentiate th PN (IBDV-positive and negative bursal tissues). Moreover, the sandwich ELISA with nAb for the detection of IBDV with mAb for the detection of IBDV is better than that with antiserum.

Keyword

ELISAFBmsal DiseaseFIBDV