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Development of Bovine Ephemeral Fever Live Vaccine Based on YHL Strain  

Update Date: [2013-11-14]
No.:45

Reporton.
AHRI report No.45
Topic
Development of Bovine Ephemeral Fever Live Vaccine Based on YHL Strain
Department
Animal Health Research Institute, Council of Agriculture, Executive Yuan
Author
YL Lee, TS Huang, CC Huang
Summary
Bovine ephemeral fever (BEF) outbreaks occur every 3-6 years in Taiwan. To improve the BEF vaccination program, we tried to develop a live vaccine using a YHL virus strain from Japan. For determining the growth rate and culture conditions of HmLu-1 cells, cell proliferation test was carried out, and the propagation curve of YHL virus was then established. As a result, the optimum monolayer of cells was found 2 days after seeding 5×10cells in 175T flask and 5×107 cells in 850 cm2 rolling bottle. After the establishment of a monolayer, culture fluid was removed and virus suspension was inoculated. Virus titer reached a peak, 107TCID50/ml in 2 days post inoculation. In the safety test, a 107.13 TCID50 YHL viral inoculation didn’t cause any fever or other side reactions in inoculated cattle during the 14 day trial. After the safety of the live YHL vaccine was confirmed, immune efficiency test of cattle was performed. The calves boosted with a dose of AHRI BEF inactivated vaccine at 3 weeks post YHL virus vaccination produced a rapid neutralizing antibody response against BEFV. The antibody titers against BEFV reached a peak of 1:128 and 1:256 within 3 to 4 weeks post boosting vaccination with inactivated vaccine and then declined gradually. These findings indicated that cattle vaccinated with a live YHL vaccine and then, boosted with a AHRI BEF inactivated vaccine showed a strong antibody response and no side effects were observed.
Keyword
Bovine ephemeral fever, Live vaccine, Neutralizing antibodies