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Publication Comparative Studies on Sulfadurg and steroid hormone preparations by...  

Update Date: [2013-11-13]

NO.: 30
 
Reportno.

AHRI report No.30

Topic

Comparative Studies on Sulfadurg and steroid hormone preparations by Hight performance Liquid Chromatography Chemical titration and Spectrophotometery.
 

Department

Taiwan Animal Health Research Institute The Brandi Institute of Animal Drugs epartin

Author P.S. Gong M.C. Li; K.M. Lin, Y.F. sheu and S.Y, Liii
Summary

Sulfamonomethoxine preparations assayi by cherrilcal titration (scdium nitrates titration with aromatic 1? amine diazotition)?UV sephotometery (UV methcd) (reacti with 3% Vanillin and detecti with U’! 397 nra) and high rerformance hquid chromatography (HPLQ (column Lichrc€orb RP-1 8, mobile phase acetonitrile:1 % acetic acid=25:73 , wavelength 280 nra). Preinisolone preparations assay&I by UV uminary methcd (reacti with eulium blire and tetramethylammoniuin hydroxide, detected with 525 nra) and HPLC (cdumn UchrcGorb RP-1 8, mobile phase methanol:H20=5 8:42, wavelength 254 nra) These methcds obtained the gcxDd result, but chemical titration and tA methcd could&t differenciate different sufadurgs or prenisolone base with its salt. Hower HPLC could solve these problem,
 

Keyword

Hight performance Liguid Chromatography, Spectrophotometry Ultraiolet Chemical titration, Glucxxorticoid steroid hormone