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Publication Identification of mycobacterium bovis and Mycobacterium tuberculosis...  

Update Date: [2013-11-13]

NO.: 35
 
Reportno.

AHRI report No.35

Topic

Identification of mycobacterium bovis and Mycobacterium tuberculosis by polymerase chain reaction

 

Department

Animal Health Research lnstitxe Conindil of Agriculture.

Author ngW,M*;Shiau,JR;WY.SLin, K.F
Summary

For the idenUlcation of inycobuctrium bvis (MB) and rnycobcerium takerculosis (Mt), Twenty nine jximers were designed and used in polymerase chain reaction (PCR), Fivereferencestains of Mt onesttofMb fourstrains of Mb B03, six stains of atypical Mycr*ectrium spp were tesel, Among thc€e five pairs of priiners?rnplM (Al )?mpk&1(A2)?npI(A3)?lS986(A1)?lS1O81 (A2)? identified Mt, but not reference strains of Mb Mb BOG and atypical MywItrium spp. In addition, Mt MB, MB BOG and MB isolates, but not atypical Mycolaterium spp were identifier! with three pairs of primers?mpb?O(A1 )?npI1(A4) and 1S986 (P1)?. Two sets of primers?mpb’?O(Al) and LS986(PTflwere used in one reaction tiLe for diagnosis of clinical sreimens (bacteria) from lymph ncdes of bot The sensitivity of the test is 61 .4%(35f3?) and the sthficity is 103% (3202).
 

Keyword

Mycokacterium bovis, Mycobacterium tuberculosis complex, PCR.