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Publication A SIMPLE METHOD FOR THE PRODUCTION OF ANTI-CHICKEN IgA ANTISERUM  

Update Date: [2013-11-06]

NO.: 24
 
Reportno.

AHRI report No.24

Topic

A SIMPLE METHOD FOR THE PRODUCTION OF ANTI-CHICKEN IgA ANTISERUM

Department

1.Natioml Taijei Cc&ge Of Nursing
2.Animal Health Research lirstitute, Council of Agriculture

Author P.H. Marl Andxew C.Y. Fei 2
Summary

Fifty ml of raw chicken bile collected from gall bladder was directly dialyaEd against acetate buffer, pH 4.6. The dialyad bile was centrifuged at S,CU)rprn for 30 mm, the surernant was applied on the DEAE column to get crude IgA without 1$) and 1gM under the buffer of 0,3M NaC1 osmolarity. The JgA in the crude IgA material was ncubatl with anti<hicken JgG antiserum. The antiuight chain antibody in the rabbit anti-chicken IgG antibody was gecipthterl with the light chain of IgA in the crude IgA material, Insoluble lattice of lthgt chain of IgA and rabbit anti-chinken light chain antibody was harvestu). by simple centrifigation, The precipitate of the lattice, aft washed with PBS for 3 times, was hnmuntd to rabbit. The antidattice, antiserum thus cosisted anti- IgA heavey chain and light chain. Aft ran throulig the Sepharose CL-4B conjugated with pure chicken 1gM, the elutl material contained rabbit anti-thicken IgA heavy chain.

Keyword

1gA;1gG ;IgMtden