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Publication COMPARISON OF ANTIGENIC COMPOSITIONS OF DIFFERENT SEROTURES OF SWINE...  

Update Date: [2013-11-07]

NO.: 25
 
Reportno.

AHRI report No.25

Topic

COMPARISON OF ANTIGENIC COMPOSITIONS OF DIFFERENT SEROTURES OF SWINE HAEMOPHILUS PLEUROPNEUMONIAE

Department

Taiwan Animal Health Reseaxch Institute

Author Wei-Ming 2*SJflow..Sijey IA
Summary

The antigenic compnents of eight serotyres of Hmophulus p]europneumoniae (HP) were analyzed by sodium dodeDyl sulfate polyacrylaxnide gel electroghoresis (SDS-PAGE) and Wean immunoblotting, There were about 38 bands and more than 45 bands of each lyre revealed in SDS-PAGE with Ceomassie blue and silver sin, reseDtively. However, there was no significant difference among serotyes. The conpceitions of difference serotyre of HP were very similar. In Western blotting, there were 30,15 and 13 antigenic bands of each strain, reseAvely, when the blotting rrrofile reacted with antisera against whole cells, serum from pig naally expDsed to HP and serum against purified lipcpo1ysaccharides (LPS). From the tterns obtained in Western blots, it was suggest] that the surface antigens played a ma role in govoking immune resronse. There were two acldtional antigenic bands, 43K and 20K of serotye 1 showed in Western blots, reacting with antisera against serotyre 1. They were concluded to ire sertye-sjtific.

Keyword

Haemophilus pleropneumoniaeSDS-PAGEerotye