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Application of Calcein in Loop-Mediated Isothermal Amplification Reaction  

Update Date: [2013-11-14]
No.:46

Reporton.
AHRI report No.46
Topic
Application of Calcein in Loop-Mediated Isothermal Amplification Reaction
Department
Animal Health Research Institute, Council of Agriculture, Executive Yuan
Author
LH Chen*
Summary
Loop-mediated Isothermal Amplification (LAMP) has been extensively applied in the field diagnosis of infectious disease. It is convenient due to the fact that results can be rapidly generated and manually visualized. Adding calcein to the reaction mixture prior to start can enhance detection by visible color change. In this study, all of 25 μM calcein-500 μM MnCl2, 50 μM calcein-500 μM MnCl2 and 75 μM calcein-500 μM MnCl2 have been found to be suitable for visible colorimetric detection. The LAMP reaction recipe containing 25 μM calcein-500 μM MnCl2 was found to be ideal for usage in both real-time turbidity and real-time quantitative PCR tests.
Keyword
Calcein, LAMP