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Publication Detection of Waterfowl Parvovirus with Real-time Polymerase Chain Re...  

Update Date: [2013-11-14]

NO.: 41
 
Reportno.

AHRI report No.41

Topic

Detection of Waterfowl Parvovirus with Real-time Polymerase Chain Reaction

Department Animal Health Research his tite, Council of Agricialftse, Exautive Yuan
Author YPChen,MSLe,SHL
Summary

The pure of the study was to develop the assay of deettion of wazerfowl rervovnis with reai-tne pilyrnerase chain reaction by p-cceeding with the Applii Bic6ysaos 75(0 Reai-Tirne PCR Lysen coupled with SYBR ga Watfowl rervovrus was subthvk1 mto gcxDGe rvovirus and Muscovy dwz]c rervovirus. Ac*xzrding to the conserved region of GPV and MDPV reAvely, the srthfic ary primers were designed f real-tine PCR. The constti real-toe PCR assay has a cle±on limit of 3 pg. The sensitivity was 1 (0-fold higher than cannon PCR. The cevelored linear relationship Iftween concentiation of nwoleic acid and cycle numlfrs of reaction was could It the I:ese of the sndard curve of nucleic add, and further, it could It applied fc paantifying the nucleic add.

Keyword

Real-toe pdyroerase chain reaction, Watrfowl parvovirus, (Jcxe rervovirus, flLsJ w Muscovy duck parvovirus