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Publication The residual study of Apramycin in animal products  

Update Date: [2013-11-13]

NO.: 30
 
Reportno.

AHRI report No.30

Topic

The residual study of Apramycin in animal products
 

Department

Taiwan Animal Health Research Institute

Author S.3. Lee. Si. 3eng and S.C. Yang
Summary

Experiments were conducted to inveestigate the residue of apramycin (100 ppm) fed to finishing pigs for 30 days. Pigs were slaughtered after to 5 days of withdrawalperiods. Tissues and serum were sampled for apramycin detenniation. Samples of muscle and tissues were extracted with methanol then concentrated and dried. The residues were dissolved by distilled water then tested by bioautography and microbiological cylinder plate methods. Serum and urine were added lN potassium hydroxide and 25% trichloroacetic Summan- acid and through the Amberlite IRC-50(H) Column, then dined with I Nammonium solution. The eluent was concentrated and dried, dissolved by distilled water, then tested by bioautography and microbiological cylinder plate methods. The detection t-as 0.05,lggQnl), average of recovery in tissue was 70.6%, in serum was 55.7%. On day of withdrawal period, all tissues sampled were residued by apramycin, with the highest level in liver (1.Sag g) and the lowest level in muscle (0.9 ag g). On day 1 of withdrawal period, no drug activity could be detected from muscle and stomach. From day 2, no drug activity could be detected from all tissues.
 

Keyword

Apramycin, Bioautography, Cylinder plate mathod