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Publication The Studies on Actinobacillus Pleuropneumoniae:Ⅱ.Preparation of Mono...  

Update Date: [2013-11-11]

NO.: 27

AHRI report No.27


The Studies on Actinobacillus Pleuropneumoniae:Ⅱ.Preparation of Monoclonal Antibodies


Taiwan Animal Health Research Institute

Author Chang, W.M.*;Y.S. Wu$.G, YangS.Y. Lin

Aft being immunized with wholle cells of sexotye 1 of Actinob&xilus (Hacmaophilus) pleuropneunoriiae (AP), the splcen cells of SaLt mice were fused with SP2A) meyloma cells. These were 12 hyhidoma keen iso1aJ which saoreted spesoific antibcdie.s against PS when screnal by ELISA and done! by limiting dilution, However, two of thcce 12 hylridoma (B& and E4d) soretui nose antibodies than others were selaotal for further study. hi enzyme immunceleoftooflsfer blot
(EB) antibody soret& by E4d were found to react with 33k antigens of seroty 1 of PS while antibody soreter1 by Boo could react with 62K, 36K 35K and 34K, resodvely. Both antibodies had species-sthficity but not serotyp&sthficity when ted by indirect fluorescent antibodies test (IFA) and immuno-reroxidase method. About5COCFUofserotye 1 ofAPwhetherboiledomnot, cou]dkedeotl indot blot assay when re&oted with E4d antibody.


Pthiokecillus Pbrropneumoniae APflrB; Moncdonal Antibodies