Home
 /  Publication  /  Volum 24  /  Publication THE PURIFICATION OF DUCK IgG AND THE PRODUCTION OF ITS ANT...
Publication THE PURIFICATION OF DUCK IgG AND THE PRODUCTION OF ITS ANTISERUM  

Update Date: [2013-11-06]

NO.: 24
 
Reportno.

AHRI report No.24

Topic

THE PURIFICATION OF DUCK IgG AND THE PRODUCTION OF ITS ANTISERUM

Department

1.Animal Health Research Institte. Cuncil of Agriculture
2.National Taipei Gllege Of Ntnsmg

Author AnchewCY. Feil;T.S. Huaszg 1RH, Chang 1 MtheI RS. La 1.H. Mar2
Summary

Ten ducks were highly immunized with purified swine IgO. The anti-swine IgO antisenim, 350m1 from the ten immunized ducks, was xsaltl out at 40% saturation in amrnonium sulfate and then chrornatgraphal through Sephacryl 5-3(I) column. The 75 and 85 serum globulins were colleztal and ahsorlM with Sepharc6e CL-4E conjugated by swine IgG. The duck anti-swine IgG anti&dy abiorlM in the affinity column was desorkalby0.1M glycine buffer, pH 2.5. Only one pxthpitation line of the desorked duck immunoglobulin was oberved when t1 in immuncelectrophoxesis anainst rabbit anti-duck whole sewn-i antiserum. This would suggest that the immunoglobulin isoland in this manner in the duck IgG &xording t the isolation of the puedpitation line in the immuncelectophoxesis analysis, Antiduck IgG antiserum was raised by inting a rabbit with the isola duck IgG. Only one prthpiition line of the anti-duck IgG antiserum when testl in iinmuncelectrophsesb against duck whole serum.
 

Keyword

immuncektrophoresis;JgG purified swine