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Publication ELISA FOR DETECTING ANTIBODIES TO BRUCELLA ABORTUS IN BOVINE SERUM  

Update Date: [2013-11-05]

NO.: 23
 
Reportno.

AHRI report No.23

Topic

ELISA FOR DETECTING ANTIBODIES TO BRUCELLA ABORTUS IN BOVINE SERUM

Department

Taiwan Animal He3lth Researdi Institute

Author Yi-ShingWu
Summary

An enzyxne-hnked immunosorkent assay (ELLSA) was used to dent antibodies to Brl]a abcnis in bovine serum, The assay was standaxdi in terms of antigen coating condition, optimal antigen conxentration, serum and conjugate dilutions. The most optimal conditions for antigen coating was that LPS antigen was diluted to lugfml vAthcarboatebuffH9fi)followerlbycoatingat4tovernightwith0.lmlof dilul antigen. The gelatin NET solution was most effaDtive in blocking. The negative cutoff point was set at 0.40 while serum sample was dilual to 1:1(0. The relative sensitivity and sthficity of ELISA to both result of complement fizition test and bike aggglutination test were 1(0% in 22 diagnostic positive sea. The correlation ccefficients of ELISA dto these two tt were 0.92? and 0943

Keyword

ELJSA; &urefla abortus dent antibodies