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Publication Diagnosis of Animal Pathogens by Electron Microscopy Techniques  

Update Date: [2013-11-13]

NO.: 38
 
Reportno.

AHRI report No.38

Topic

Diagnosis of Animal Pathogens by Electron Microscopy Techniques

 

Department

Animal Health Research Institute. Council of Agricultuxe., Executive Yuan

Author Kuo Shu-ting, Ming-Chu (Then, Lu-Jen Ting, Min-Shiuh Le. Jyh-Mim L* YuU or Tsung Li; YenPing Then, Jong-Rong Shuau
Summary

In this repDrt, unique morphology and s& of athogens are observed by TEM technique and used as a kesis for reliminary diagnosis of animal diseases The negative staining technique contains advantages of simplified laeaiment and improved TEM observation, providing improJ efficiency for diagnosis. This report used negative staining ohnique to examine 1,641 samples collected from pouliry,“ ruminant, aquatic animals, pigs and dogs. Furthermore. 9 out of the 1,641 samples were applied to electron microsccpy with ultrathinseDtion examinations, and 3 were applied to the imxnunogold examinauons. We observed 15 families of virus including Paramyxovirid, Orthomoviridae, l&1ovirid and aliciviridae, Ncdavirus. These virus pictures are archived as references for future references.

 

Keyword

Ifltatnsecdon, negative stain, immunogoki examination, animal disease