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Publication Simultaneous determination and survey of 2, 4-diaminopyrimidines res...  

Update Date: [2013-11-13]

NO.: 33
 
Reportno.

AHRI report No.33

Topic

Simultaneous determination and survey of 2, 4-diaminopyrimidines residues in eggs by high-performance liquid chromatography

 

Department

Animal Health Research Institat, Council of Agriculture

Author Ya-Fajg Uu*S.J. Iie,C. Yand’.P. Liou
Summary

A high-performance liquid chromatographic (HPLQ method for simultaneous determination of the residue of 2, 4-diaininopyrimidine dmgs, incluthng trimethoxim (TMP), ormetoprim (OMP), and pyrimethamine (PYM) in eggs was develop. Samples were extxacti with aretonithle, centrifugal, pertitional with n-hexane, concentratd by rotary evaporation to dryness, and then analysel by HPLC. The conditions wereestablishaionau-BondapakCl8 column with SxlO-3 M acetic acid buffer/methanol as mobile phase and deil at Ii’! 230 nm, The average recoveries of the three drugs spiked into samples at the levels of 0.5,1.0, and 2.0 ug /g were 76.5 % to99.6 %. Thelowestdetaztionhmitofresidualdrugs were0.O2ug/gineggs. 3CC samples from layer farms in Taiwan ar were surveyed by random for the residues of NP, OMP, and PThI with the develorel method. The result indicati that all samples were examined to ke kelow the debztion limit of the three drugs.

Keyword

2, 4-diaminopyrimithnes HPLC; Simultaneous detenninationRnidues.