Home
 /  Publication  /  Volum 28  /  Publication High Performance Liquid Chromatographic Rapid and Simultan...
Publication High Performance Liquid Chromatographic Rapid and Simultaneous Deter...  

Update Date: [2013-11-11]

NO.: 28
 
Reportno.

AHRI report No.28

Topic

High Performance Liquid Chromatographic Rapid and Simultaneous Determination of Fat Soluble Vitamins (A. D3.E) in Premixes.

Department

Taiwan Animal Health Research Institute The Branch Institute of Animal Drugs Inspection

Author K.M. Em FM.C. LiuFK.M. LinFP.P. Liou
Summary

Premixes bulk sample was pretreated with a little volume of dimethylsulfoxide, then absolute ethanol was added to volume. After mixing and filtration, the filtrate was injected into a high performance liquid chromatography (HPLC) which was equipped with a £g-Bondapak CiS 4.01 250mm column and 280-nm detector. The mobile phase was 100% methanoL Fat soluble vitamins (A, D3, E) could be determined simultaneously. The retension time ofVit. A 100 iu ml. Vit. D3 200 iu ml. and VitE 0.15 iu ml were 42, 53. and 6.7 minutes. respectively. Alkaline saponification of the pretnixs bulk sample was found helpfUl in case of the peak of Vit. A has some interrupts or the concentration of it. A is lower By using HPLC, the fat soluble vitamins (A, D3, E) in premixs can be determined rapidly and simultaneously and the risk of exposure to toxic organic solvents, the time and man power of operation can be reduced.

Keyword

Vitamins FSimultaneous DeterminationjHPLC