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Publication Case report : Biological properties of Lactococcus garvieae isolated...  

Update Date: [2013-11-13]

NO.: 33

AHRI report No.33


Case report : Biological properties of Lactococcus garvieae isolated from diseased rainbow trout



Animal Health Research Institute, Council of Agriculture

Author CHEN Chingt$.J. ko;LP, Chanfl. Liou

During the months of March and Agil of 1996, about four to five hundreds of rainbow tout died in an aquacultrne pond, about one hectare in si and cultured 120- 130 thousands of rainbow bouts, lcca& in the northern part of Taiwan. The symptoms on the outlooks were hemorrhaged eyeballs with ulcer in the surrounding areas. Autopicral examinations found degenera1 liver color, enlargement of bile bladder, dark spleen, and kidney enlargement and darkness. The iso]atal bacteria were cultured with blocd agar, brain heart infusion agar, tryptic soy agar, and SS aga.r. They grew in the first three media at 25? and 3’?? but not at 45?. On the contraq, the bacteria did not growth in SS agar at all under the three different tmrerawre conditions. The colonies on brain heart infusion agar were uniform in si and appeared with milk white color.
They showed no hemolysis on blood agar culture. Gram were positive by 3 % KOH examination; catalase and oxidase test were all negative. In light miaoscope examination, they appeared like bioxcus or short chain and did not have a flagellum. Mditional obervations by electron miaoscqe found pu-like subatance. According to the API 32 Stsept tests, Lactccxxcus garvieae was identified. When sthogenicity tests using mice and Cyinus carpio were conduced, the bacteria could not cause mortality in either case. The antibiotic sensitivity test performed revealed that the bacteria were more sensitive to ampicillin and erythromycin than amikacin, chioramphenicol, clilortetracycline, lincoinycin, gentanricin, rifarupicin, tetracycline, trimethoprirn, nalidixic acid, nitofurasrtoin, kanamycin, oxyn-&zydine and steptomycin. This is the frist report of & Lactococcus garvieae isolated from rainbow tout in Taiwan.


Rainbow toutHemorrhageLactooxcus garvieaeAxnpidThn; Amoxicillin.