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Research Information Development and application of recombinant proteins of porc...  

Update Date: [2013-11-01]

Plan Year: 2005
 
    Recently, porcine circovirus type2 represents the causative agent of a new porcine disease which spread in form of an epidemic all over the world in the past years.  This study is to extracte viral DNA was from Taiwanese PCV2, ORF1- and ORF2-encoding region was amplified by PCR.  The PCR product was inserted into the different vector, expression and detected with anti-PCV2 polyclonal antibody by Western blotting.  Recombinant ORF1 and ORF2 protein will be purified with HisTrap column, and coated on the well surface of 96-well ELISA plates.  The antibodies titers of the sera from different farms and age groups are determined by ELISA.  On the other hand, real-time PCR will be application to detect antigens of PCV2 in serums.