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Investigation of Toxoid Inactivated Bacterin against Actinobacillus pleuropneumo...  

Update Date: [2013-11-14]
No.:47

Reporton.
AHRI report No.47
Topic
Investigation of Toxoid Inactivated Bacterin against Actinobacillus pleuropneumoniae
Department
Animal Health Research Institute, Council of Agriculture, Executive Yuan
Author
NL Kuan, HH Tsai, CS Huang, CC Wu, C Tu
Summary
The trial inactivated bacterin was composed of 1 × 108 CFU / ml for each serotype (AP1, AP2, AP5 and AP7), 10x concentrated toxoid ApxI and adjuvant ISA 25 (O / W). The trial vaccine was passed the safety tests in mice, and the defense index (DI) for serotype 1, 2, 5 and 7 were 1.3, 1.0, 1.5 and 1.0.  A high dose (5 dose; 2 ml / dose) was injected i.m.as the safety test in pigs, except the injection site swelling, no other abnormalities were observed. After challenging with 5×107 CFU serotype 1, the vaccinated group was survived and recovered in 72 hours, whereas the control group was died in 24 hours. The hemolysis inhibition assay and ELISA kits were used for antibody detection, the ApxI antibodies and AP antibodies were ascending after boost, however, the seroconversion rates of serotype-classified antibodies were 0-100%, suggesting that the variation existed between isolates even in the same serotype. All of three batches of the inactivated bacterin were qualified in safety tests, and one batch was disqualified from efficacy tests.
Keyword
Actinobacillus pleuropneumoniae, toxoid, inactivated bacterin