Home
 /  Publication  /  Volum 23  /  Publication A SIMPLE METHOD FOR THE PRODUCTION OF ANTI-CHICKEN IgA ANT...
Publication A SIMPLE METHOD FOR THE PRODUCTION OF ANTI-CHICKEN IgA ANTISERUM  

Update Date: [2013-11-05]

NO.: 23
 
Reportno.

AHRI report No.23

Topic

A SIMPLE METHOD FOR THE PRODUCTION OF ANTI-CHICKEN IgA ANTISERUM

Department

Taiwan Animal Health Reseaxch Institute

Author Andrew Chang-Yung Fei
Summary

Fifty ml of chicken bile was initially dialyzed against aqainst butter followal by buffered saline, and chxoxnatgrap]M on DEAF dialuzed column. The majtdty of bi]ioary IgA was eluel with O,3M NaQ and further dialyd against PBS. The crxie bihary IgA was peclpitaed wtih rabbit anti-chicken IgA anten, The waterinsolubIe lattice nipitate was washer! with PBS. Rabbit anti-chicken IgA (H-i-L) anti-semrn was gcdwei by the infrttion of rabbit with the washed lattice aipitue emulsifier! withadjuvanis. The antiserum was aborlerlwithSepharoseCL-4B conjugaelby pure 1gM to obtain anti-IgA (H): antiserum.

Keyword

ANTI-CHICKEN IgA ANTISERUM ; chicken bile; crude biliary