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Seminar 872  
Date:2017-04-20
Update:2017-11-06

  

Seminar:  872  

1

Speaker(s)

Yi-Ping Lu

Topic

Diseases monitoring and diagnosis of ornamental fish from 2015 to 2016

Abstract

Taiwan is well-known internationally for it aquarium animal breeding and propagation industry. In total, the Taiwanese aquarium trade reached up to 5 billion New Taiwan Dollars (NTD) by the end of 2014, and is estimated to reach 7.5 billion NTD in 2017. It is one of the most competitive high-end agriculture industries in Taiwan. To enhance the competitiveness of the Taiwanese ornamental fish industry in the international market, we provided the aquaculture operations located at the Pingtung Agricultural Biotechnology Park with routine sampling and monitoring of 15 kinds of important diseases listed in the OIE table (seven fish and eight crustacean diseases) from 2015 to 2016. Disease monitoring was conducted in 113 aquaculture farms in 2015, and 107 in 2016. A total of 38,356 samples (8,503 samples for ornamental fish and 29,853 samples for ornamental shrimp) were examined with molecular biological assays. In ornamental fish, the highest observed disease prevalence was 1.12% (19/1695) for Koi herpesvirus (KHV) and 0.21% (26/12403) for red seabream iridovirus disease (RSIVD). In ornamental crustaceans, the highest observed disease prevalence was 0.31% (18/5857) for white spot syndrome virus (WSSV) and 1.78% (61/3428) for infectious hypodermal and hematopoietic necrosis virus (IHHNV). For aquatic animal disease diagnosis, 315 cases were received (133 in 2015; 182 in 2016) over the past two years. Currently, 13 ornamental aquatic animal farms (five koi farms; eight shrimp farms) have been certified to be free of diseases through this 2-year monitoring span.

2

Speaker(s)

Shu-Chia Hu

Topic

Monitoring of coronavirus in bats of Taiwan in 2016

Abstract

Since the global outbreak of the deadly SARS and MERS coronaviruses (CoV), the importance in understanding the origins of these viruses in wildlife and how they are transmitted to humans has been highlighted.. Bats have been recognized as the reservoirs of many zoonotic viruses, including coronavirus. Coronaviruses have been identified in more than 100 bat species. The coronavirus monitoring project was established in cooperation with the Bat Conservation Society of Taipei and local disease control centers in 2016. In total, 102 carcasses and 19 fecal samples from 7 bat species were collected, and coronavirus was detected in 5 samples. Phylogenetic analysis of the partial RdRp gene (RNA-dependent RNA polymerase) indicated that 2 of the samples harbored alphacoronavirus, while the other 3 samples harbored betacoronavirus. The results of this monitoring project and associated research projects all indicate that coronaviruses have been detected amongst the bat population of Taiwan. To ensure continued public health and safety, sustained monitoring of coronavirus in wildlife is necessary.

3

Speaker(s)

Ling-Chu Hung, Chu-Hsiang Pan, Chun Wang

Division of Hog Cholera Research

Topic

Investigation of swine health on pig farms through the effective monitoring of porcine diseases and immunological response to vaccination

Abstract

This study is ultimately focused on reducing the risk of swine disease with an emphasis on prevention, diagnosis, and treatment. According to each pig farm’s epidemic prevention needs, we provided customized diagnostic tools to establish secure rearing practices, including the use of advanced immunization, molecular diagnostic methods, and the rapid provision of effective epidemic prevention measures. A total of 1492 serum samples (28 batches) tested positive for viral antibodies in this study, including 1492 samples for porcine pseudorabies (PR), 308 samples for porcine reproductive and respiratory virus (PRRSV), 308 samples for porcine circovirus type 2 (PCV2), 308 samples for hog cholera (HC), and 26 samples for porcine epidemic diarrhea virus (PEDV). Furthermore 42 samples tested positive for viral infection using standard PCR screenings. We detected one positive sample for coronavirus, 7 positive samples for PCV2, 12 positive samples for PRRS, and 16 positive samples for PEDV. In addition to using serum neutralizing antibody tests, this program also utilized commercially available kits for the detection of specific antibodies. Moreover, we used molecular biotechnology methods to produce recombinant proteins for coating antigens for ELISA. These methods enabled us to detect more specific antibodies in order to more precisely immune response to the various vaccination programs. In addition, the use of customized diagnostic services and standard blood collection efforts can be an effective monitor of infection as well as provide an effective observation of antibody titer distribution curves. The optimal age of inoculation was estimated by assessing the risk of a specific disease or the immune response after vaccination.