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Seminar 848  
Date:2015-04-08
Update:2015-05-02

Seminar: 848

1

Speaker(s)

Yeou-Liang Lin

Topic

The report of detecting antibodies against Avian Influenza H9N2 Virus in Taiwan

Abstract

Due to the report of Hong Kong University professor, Guan Yi, published a paper in the Journal of Virology about the antibodies against avian influenza H9N2 virus inTaiwan. A batch of chicken sera collected in Taiwan poultry slaughterhouse was rechecked. The chicken serum samples, including 100 serum samples were collected at the same poultry slaughterhouse, Taipei Poultry Market, on April 15, 2014 and 1490 serum samples were sent by Poultry Health Center Central Laboratory, National Animal Industry Foundation and were collected between January 2014 and July 8, 2014 basing on the active surveillance project which have been prior detected antibodies positive against the H5 and H6 of avian influenza virus, were implemented the hemagglutination inhibition (HI) test by using two stains of avian influenza H9N2 viruses (A / CK / HK / HH117 / 2012, HK-HH117 and A / CK / HK / NT155 / 2012, HK-NT155) which were shared from the laboratory of Professor Guan Yi in Hong Kong University for the detection of antibodies against H9N2 viruses respectively. The results of the 100 serum samples from Taipei Poultry Market demonstrated that only two serum samples from a guinea fowl farm to HK-HH117 AIV showed seropositive but with low antibody titer; on the contrary, to the HK-NT155 AIV was seronegative. The results of 1490 serum samples from the active surveillance project indicated that all of the serum samples to HK-HH117 AIV were shown seronegative; whereas to the HK-NT155 AIV have 7 serum samples shown seropositive (antibody titers were greater than 1: 16). These antibody-positive sera were collected from five different farms (four chicken farms, one layer farm).

2

Speaker(s)

Cheng, Ming Chu

Topic

Evaluate the effect of avian influenza vaccine containing H5N2-VLP as antigen and rVP3 as adjuvant

Abstract

Avian flu is a major disease in poultry. The disease is caused by influenza A virus. Highly pathogenic avian influenza resulted in the malignant spread and high mortality. Most of the countries adopted culling strategy to control the disease and restricting the use of vaccination. This cooperative research program aims to overcome the problem of avian influenza vaccine use. The efficacy of the H5N2-VLP and rVP3 as potential vaccine was examined. The data revealed that chicken immunized with H5N2-VLP alone did not elicit sufficient antibody titer suggesting that H5N2-VLP requires assistance of adjuvant(s) to produce full immune responses. In comparison, chicken primed and boosted with 3 mg H5N2-VLP together with rVP3 and alum as adjuvant elicited high antibody titers, fully survived from virus challenge and yielded less virus shedding than inactivated whole virus vaccine. Most interestingly, chicken immunized with H5N2-VLP in the presence of rVP3 and alum as adjuvant elicited higher amounts of IFN-g, IL-18 and IL-10 gene than H5N2-VLP adjuvant with alum alone.

3

Speaker(s)

Kuo shu ting

Topic

2014 Electron microscopy work report

Abstract

Due to the report of Hong Kong University professor, Guan Yi, published a paper in the Journal of Virology about the antibodies against avian influenza H9N2 virus inTaiwan. A batch of chicken sera collected in Taiwan poultry slaughterhouse was rechecked. The chicken serum samples, including 100 serum samples were collected at the same poultry slaughterhouse, Taipei Poultry Market, on April 15, 2014 and 1490 serum samples were sent by Poultry Health Center Central Laboratory, National Animal Industry Foundation and were collected between January 2014 and July 8, 2014 basing on the active surveillance project which have been prior detected antibodies positive against the H5 and H6 of avian influenza virus, were implemented the hemagglutination inhibition (HI) test by using two stains of avian influenza H9N2 viruses (A / CK / HK / HH117 / 2012, HK-HH117 and A / CK / HK / NT155 / 2012, HK-NT155) which were shared from the laboratory of Professor Guan Yi in Hong Kong University for the detection of antibodies against H9N2 viruses respectively. The results of the 100 serum samples from Taipei Poultry Market demonstrated that only two serum samples from a guinea fowl farm to HK-HH117 AIV showed seropositive but with low antibody titer; on the contrary, to the HK-NT155 AIV was seronegative. The results of 1490 serum samples from the active surveillance project indicated that all of the serum samples to HK-HH117 AIV were shown seronegative; whereas to the HK-NT155 AIV have 7 serum samples shown seropositive (antibody titers were greater than 1: 16). These antibody-positive sera were collected from five different farms (four chicken farms, one layer farm).