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Seminar 809  

Seminar:  809

1

Speaker(s)

Epidemiology Division1, Biologics Division2, Hog Cholera Division3

Shu-Hwae Lee1, Tien-Shine Huang2, Yeou-Liang Lin3, Chun Wang3, Yu-Ju Lin2, Jen-Chieh Chang1

Topic

2011 Report on “Taiwan-Vietnam Bilateral Conference on Technical Collaboration in Swine Medicine” and round-table discussion on “Taiwan-Vietnam Bilateral Livestock Academic Cooperation” in Vietnam

Abstract

Animal Health Research Institute (AHRI) organized a group and cooperated with Nong Lam University (NLU) to hold “2011 Taiwan-Vietnam Bilateral Conference on Technical Collaboration in Swine Medicine”. The twenty representatives of the Taiwan group were from AHRI, Breeding Swine Industry Association of Taiwan, College of Bioresources of National Ilan University, Department of Animal Science of Chinese Culture University, Chemical & Pharmaceutical and other related companies. Both Taiwan and Vietnam experts gave five lectures and the number of participants was about eighty. The Vice Director of Department of Animal Health, Vietnam, Mr. Mai Van Hiep and the Rector of NLU, Dr. Trinh Truong Giang were invited and they did pay much attention to this conference. For further Taiwan- Vietnam technical collaboration, we also held a session of round-table discussion on “Taiwan-Vietnam bilateral livestock academic cooperation” in NLU. The subjects “Studies on the pathogenesis, diagnosis and prevention method of important viral diseases of pig”, “Application studies on the pig disease diagnostics developed by Taiwanese scientists in Vietnam laboratory”, “The application study on the LPC-China CSF vaccine in the pig farms in Vietnam”, “International cooperation and academic exchange activities between our colleges (CCU and NIU) and NLU” were discussed how to promote possible cooperation in the future. The representatives of NLU expressed high appreciation to this Taiwan-Vietnam communication. They agreed that both side of Taiwan and Vietnam should prepare to sign technical Memorandum of Understanding (MOU). That would be helpful to research cooperation, development and popularization of vaccines and diagnostic reagents. We also visited NLU and Kimlong breeding pig farm to understand the situation of veterinary education and pig production.

 

2

Speaker(s)

Wei-Cheng Hsu

Topic

2011 report on OIE global conference on rabies control/ surveillance on rabies in Taiwan

Abstract

The “2011 global conference on rabies control”, held in Seoul, Republic of Korea, had attracted over 300 participants from 85 countries. The purpose of the conference is to offer a communication platform and encourage participants to share rabies control experience with each other. By attending the conference, we could update the current global rabies epidemiologic situation and the modern rabies studies trend. The information we got had huge contribution to strengthen rabies control and prevention strategy in Taiwan. The rabies surveillance program was started since 1999 and 1,796 animal samples were tested in 2011. The fluorescent antibody test (FAT), histopathology, and RT-PCR were used to detect rabies virus antigens. In rabies antigen surveillance, we divided all cases into three groups by animal sources and risk status: (1) routine surveillance: 1,186 brain samples of weak or dying dogs were collected from shelter houses; (2) rabies suspected cases: 11 samples (including 1 oral swab) of dog eliciting signs of nervous syndrome or those with a history of biting people were sent to us by local Livestock Disease Control Center; (3) wild animal surveillance: 37 samples of sick or dead bats were collected by cooperation with Bat Conservation Society of Taipei. Rabies viral antigen and typical lesion were not detected in all samples. Meanwhile, 562 serum samples from domestic dogs were examined by the enzyme-linked immunosorbent assay (ELISA) for antibodies against rabies virus. A minimum of 0.5 IU/ml rabies antibodies is required for protection against rabies infection, according to the World Health Organization (WHO) recommendations. Results showed that the sero-positive rate of domestic dogs was 57.1 % (321/562). Our study also indicated that the free status of rabies maintains in Taiwan, and more attention on wild animal and imported animal surveillance in the future is necessary.

3

Speaker(s)

Tsu-Han Chen

Topic

Development of ELISA for detecting antigens of foot-and-mouth disease virus

Abstract

The aim of the this study was to develop a sandwich enzyme-linked immunosorbent assay (sandwich ELISA) which can detect antigen of foot-and-mouth disease virus (FMDV). Anti-FMDV monoclonal and polyclonal antibodies were derived from either recombinant FMDV protein or whole inactivated FMDV. The antibodies were labeled either with horseradish peroxidase or biotin and were applied to the ELISA for antigen detection. Our results indicated that both monoclonal and polyclonal antibodies were able to capture the antigens in samples and showed a clear distinction and no cross reaction between serotypes. Analytical specificity of the ELISA in seven serotypes of inactivated FMDV was high. For applying to the FMDV detection, including analytical sensitivity of RT-PCR, commercial chromatographic strips, and our ELISA , the data were shown 10-6, 10-3 and 10-3, respectively. The results indicated that the ELISA can detect FMDV antigen in the samples and does not react with SVDV antigen.