Home
 /  Seminar  /  Seminar 828
Seminar 828  
Date:2013-08-14
Update:2014-08-12

 

Seminar:  828

1

Speaker(s)

Ai-Ping Hsu

Topic

Development of Newcastle Disease Live Attenuated Vaccine

Abstract

Newcastle Disease (ND) is an important avian viral disease causing serious economical damage in the world. Antiginicitiy divergence exists between Newcastle disease virus (NDV) of different genotypes, and at present the main circulating and endemic field strains all belong to genotype VII in Asia countries such as Taiwan, China, Korea, and even in Europe and Australia, those result in the ND live vaccines of genotype I or II in the market could not offer ideal protections. The animal vaccine development team of AHRI (Animal Health Research Institute, Council of Agriculture) has successfully developed a ND live vaccine of genotype VII— 95NDV-E15, which was derived from attenuation procedures for more than 100 passages not only in embryonated eggs and cells, but also adapted to strict temperature from a virulent wildtype NDV. 95NDV-E15 exhibited ICPI (intracerebral pathogenicity index) of 0.143 and IVPI (intravenous pathogenicity index) of 0. For evaluating safety of 95NDV-E15, 1, 10, or 100 doses of 95NDV-E15 were inoculated into 1-day-old chicks by intranasal/eye-dropping routes, and all chicks were healthy for the 21 days. When compared with commercial vaccine, 95NDV-E15 could offer better protection. There were 31 nucleotides different from the virulent wildtype NDV out of 15,192 nucleotides in full-length of 95NDV-E15 genome, and the phylogenetic analysis demonstrated they were all classified into the same cluster of genotype VIId. In the future, by using reverse genetics to construct recombinant NDV, the virulence-decline mechanisms will be investigated.

2

Speaker(s)

Jen-Huan Tsai

Topic

Veterinary Service Satisfaction and Educational Training Model Research

Abstract

In order to understand our institute education and training effectiveness and satisfaction. we survey four categories questionnaire in 2012. 1. 12 times regular seminar questionnaire survey, issued 525 copies of questionnaires, and returned 374 (response rate 71.24%), the average satisfaction rate of 90.68%; 2. 10 times veterinarians and farmers development education train, issued 710 copies of questionnaires, and returned 261 (response rate 36.75%), the average satisfaction rate of 89.7%; 3. student dormitory satisfaction survey in 2012 issued a total of 272 copies of questionnaires, and returned 209 (response rate 76.84%), the average satisfaction rate of 88%; 4. Our research reports and newsletter readership satisfaction survey, issued 944 copies of questionnaires, and returned 158 (response rate 16.74%), the average satisfaction rate of 95.44%.

We hold "Farmers Academy - swine diseases and epidemic advanced elective training class" on June 4 to 6 in 2013. We chose 30 pig farmers or resident veterinarian to receive a intensive training. We based on the Coriolis four level training evaluation model for response assessment and learning assessment. In the future, we will carry on the post-training behave and outcome evaluations, in order to track and evaluate the effectiveness of this training.