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Seminar 843  

 

Seminar:  843

1

Speaker(s)

Ai-Ping Hsu

Topic

Development of Newcastle Disease Virus Vector Vaccine, Study of Rabies Virus Reverse Genetics Technique in Gifu University of Japen and Report of Attending IUMS Conference in Canada

Abstract

Newcastle Disease (ND) is an important avian viral disease causing serious economical damage in the world, so the use of Newcastle disease virus (NDV) vaccine is necessary in every poultry farm. By using reverse genetics techniques, Development of NDV vector vaccine has been a current trend, and its commercial product even shows in the market at present. We has successfully developed an NDV live vaccine of genotype VII at AHKI and further, the reverse genetics platform has been established. In the future, antigenic fragments derived from other pathogens will be constructed into the backbone of our live NDV vaccine for multivalent vaccine development. As both rabies virus (RABV) and NDV belong to order Mononegavirales ,nonsegmented negative stranded RNA viruses, the reverse genetics manipulation of two viruses are highly similar. Through visiting and consulting Laboratory of Zoonotic Diseases of Gifu University in japen that has set up reverse genetics platform on rabies virus for many years, our NDV vector vaccine development study can be improved. At the end of the seminar, the experience and information from attending International Unit of Microbiological Societies (IUMS 2014) conference in Canada will be introduced as well.

2

Speaker(s)

Nan-Ling Kuan

Topic

Prevalence of Antimicrobial Resistance of Avian Salmonella

Abstract

Avian Salmonella infections cause acute or chronic diseases in poultry, which are responsible for large economic losses in the poultry industry worldwide. The etiological agent of pullorum disease and fowl typhoid is Salmonella enterica subsp. enterica serovar Gallinarum, which is divided into two distinct biovar, Pullorum and Gallinarum. Drug resistant strains might be caused by increasing use of antimicrobial agents for disease treatment or prevention. As an important mechanism of antimicrobial resistance, integrons refer to mobile DNA elements, which may contain one to several resistant gene cassettes. In this study, 21 isolates of Salmonella Pullorum and 4 isolates of Salmonella sp. from poultry were included. The results revealed that 60% (15/25) of isolates carried integrons with various size: 721 bp, 1,000 bp, 1,600 bp and 1,900 bp. These integrons contained gene cassettes encoding resistance to aminoglycosides (aadA1 and aadA2) and trimethoprim (dhfrA12, dhfrA25 and dhfrI). All of the 7 dhfr-positive isolates were resistant to trimethoprim/sulfamethoxazole; the 14 aadA-positive isolates were increased in the minimum inhibitory concentration of streptomycin. The results indicated that types of resistant gene cassettes reflected the relevant resistances in Salmonella isolates.

3

Speaker(s)

Tzu-Ming Huang

Topic

Diagnosis of Escherichia coli in Postweaning Diarrhea in Pigs and Meeting Report: 7th International Symposium on Aquatic Animal Health

Abstract

Escherichia coli is one of the most important causes of postweaning diarrhea in piglets. The objective of the present study was to investigate the E. coli isolated from the piglet diarrhea cases submitted to Bacteriology laboratory. Up to date, 47 cases were submitted to Bacteriol. lab. in 2014. Among them, the complaint of 12 cases was piglet diarrhea. E. coli was isolated from all cases, except one case only requested for Clostridum isolation. By molecular detection for enterotoxin gene, the E. coli isolates from 7 cases were negative, 2 cases were positive for heat labile enterotoxin (LT), 1 case was positive for heat stable enterotoxin (ST), and 1 case was positive for LT, ST, and Shiga like enterotoxin (STx). The antimicrobial susceptibility of the LT/ST/STx E coli isolate showed no sensitivity to beta-lactam drugs, 3rd cephalosporins, quinolones, tetracycline and sulfonamides. The results of the present study revealed that enterotoxin producing E. coli could be isolated from the piglet diarrhea outbreaks in 2014. It was suggested to review the treatment and control policy of neonatal colibacillosis, and to monitor the enterotoxin producing E. coli distribution for public health protection.

Ms. SM Huang and I attended the 7th International Symposium on Aquatic Animal Health in Portland, OR, USA in August 31 to September 6. SM Huang presented a poster titled “Development of an immunochromatographic test kit for rapid detection of fish iridovirus”