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Seminar 821  
Date:2013-01-19
Update:2014-08-12

 

Seminar:  821  

1

Speaker(s)

Shu-Ting Kuo

Topic

Non-specific substances in cell culture medium observed by electron microscopy

Abstract

Since October 2012, many problems can not be solved in the process of cell culture. Cell morphology abnormal or even death without inoculated any pathogen.Proliferation of the virus can not be in this cell.Culture medium observed 25 ~ 28 nm size of the particles, but after other tests can not prove the specificity of the particles. Repeatedly detecting observed fetal calf serum, are added in the culture medium, also similar to the size of the non-specificity of the presence of particles. Different brands of fetal calf serum, the number of particles amount different. Speculated that this particle may be non-pathogenic substances. By replacement of fetal calf serum, the cell culture situations have improved.This seminar is the report of the cell culture medium observed by electron microscopy.

2

Speaker(s)

Chao-Fang Yu

Topic

Development of Riemerella anatipestifer Bacterins and Its Egg Yolk Antibodies

Abstract

With previous developing experience, the study is to develop another trivalent inactivated bacterin against Riemerella anatipestifer infection in order to cope with variant serotypes infection in the field. The three monovalent bacterins, serotype 9, 14 and 17, as well as these combined trivalent bacterin have been found to be efficacious against homologous challenge on ducks and geese. In addition to vaccination, ducks and geese administered with the corresponding egg yolk antibody against the prevalent serotype infection could quickly obtain urgent protection at their susceptible age. The IgYs in WSFs (water soluble fraction) from the eggs laid by chicken or duck layers hyper-immunized with serotype 2 bactern, were given to ducklings or goslings and challenges followed. The results showed that both chicken WSF and duck WSF could provide effective passive protection against homologous challenge on ducks and geese.

3

Speaker(s)

Ming-Chu Cheng

Topic

Avian Influenza virus surveillance in Wild Birds in 2012

Abstract

The objective of this report is to analysis the surveillance data on avian influenza virus (AIV) in wild birds in 2012.  In 2012, a total of 4,395 specimens collected from the wild birds in Taiwan for active and passive surveillance. Nineteen AIVs were isolated from waterfowl specimens on active surveillance. The highest monthly prevalence was in January (1.08%). The yearly prevalence of active surveillance on waterfowl of this year (0.45%) was lower than last year (0.72%) and average of before last year (0.81).  Most of AIVs were isolated from the specimens of duck. The prevalence in ducks is 0.60%, lower than last year (1.09%) and average of before last year (1.08%).  All of the isolated AIVs were distributed into 5 different H/N subtypes; H1N1, H1N3, H4N6, H7N1 and H10N3. Most isolates were belonged to H7N1 subtype (fourteen in 19 isolates). H7 viruses were isolated in 5 different sample of sampling times.  These viruses were identified as the nonpathogenic strains by the results of the amino acid at cleavage site of hemagglutinin protein. In the results indicated that, no any highly pathogenic avian influenza viruses had be fund in the specimens of active and passive surveillance.

4

Speaker(s)

Tsung-wen, Hsu

Topic

Evaluation the effectiveness of the training for the pig farmers in the swine production medicine

Abstract

The project is aimed to evaluate the effectiveness of the training for the pig farmers about the swine production medicine and bio-security . The training evaluation model was established in scientific methods.

The trainees took the questionnaire which dimensions include training courses response, learning result, training behavior and the improvement of the effectiveness of the work to evaluate the performance of the swine production medicine courses and our veterinary technical service. The survey issued 115 questionnaires from June27 to August 28, and recovered 49 (overall response rate at 42.61).

According to the analysis, most of the response to the training courses was satisfied. But the curriculum planning and learners recruitment in the future should be more strengthened in order to avoid the gap between trainees’ competence and the course content. The survey of learning results showed that they could meet business partners, obtained the solutions to the pig farm problems and new knowledge and concepts of health management of pig farms. The changes of the training behavior indicated that the trainee improved and executed the health management and bio-security measures of the pig farm and were willing to accept the advanced training courses. The improvement of the effectiveness of the work exhibited enhancing trainee self-confidence, work opportunities and production efficiency. The effectiveness of the overall training fit in with the goal of the training course.