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Detection of Classic Swine Fever Virus by Application of Loop-mediated Isotherm...  
Date:2007-02-15
Update:2013-12-26

Seminar:  Detection of Classic Swine Fever Virus by Application of Loop-mediated Isothermal Amplification method

1

Speaker(s)

Ming-Chung Deng

Coordinator

Shu-Hwae Lee

Abstract

Recent advances in molecular biology have greatly improved the nucleic acid amplification techniques in which amplification of specific nucleic acid sequences becomes more convenient since polymerase chain reaction (PCR) has been invented. However, the common PCR technique needs specially designed thermal cycler and well-equipped laboratory to carry out the following experiments. It becomes difficult and time consuming to make disease diagnosis in the fields when there is no specific equipment and laboratory, easily leading to disease outbreak. Therefore, numerous scientists have developed several reliable and convenient techniques for nucleic acid amplification to detect virus nucleic acids. Isothermal amplification techniques were thus developed including nucleic acid sequence bases amplification (NASBA), strand displacement amplification (SDA), and loop-mediated isothermal amplification (LAMP). The LAMP technique needs less equipment and are more convenient to carry out compared to other techniques. Therefore we hoped to develop a method for rapid detection of the specific sequence of classical swine fever virus (CSFV) nucleic acids using LAMP technique. After mixing the extracted nucleic acids with specific primers, the reaction was carried out under the certain temperature and then the electrophoresis test was made. The results from the above experiments suggested that LAPM technique can be successfully applied in the detection of classical swine fever virus. The development in the detecting technique for CSFV by applying LAMP may profoundly help the establishment of rapid field diagnosis of CSFV and prevention of disease outbreak.