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Seminar 844  

 

Seminar:  844  

1

Speaker(s)

Shao-Hui Cheng

Topic

Antimicrobial susceptibility of Vibrio spp. in aquatic animals

Abstract

Vibrio species are normal flora and important pathogens in aquaculture. To analyze antimicrobial susceptibility of Vibrio spp., 41 Vibrio isolates from aquatic animals were collected from southern Taiwan. Disk diffusion assay and minimum inhibitory concentration (MIC) were used for characterization of antibiogram of the isolates. Eighteen isolates were resistant to ampicillin (18/41, 44%), 3 isolates were resistant to tetracyclines (3/41, 7.3%), 4 isolates were resistant to sulfamethozazole/ trimethoprim (SXT) (4/41, 9.7%), and 4 isolates were resistant to oxolinic acid (4/41, 9.7%). The resistant determinants of beta-lactam drugs including blaSHV, blaTEM, and blaCTX , and those of tetracycline including tetA-E, tetM, tetS, tetG, tetY were detected by PCR. Detection of class 1 integron was performed in SXT resistant isolates by PCR. The result showed that blaSHV was detected in 1 isolate among 18 ampicillin resistant isolates; tetD and tetM genes were simultaneously found in 2 isolates among 3 tetracycline resistant isolates. Class 1 integron was found in 3 isolates: 2 of them carried a 1kb-cassette (aadA8), and the other carried a 2kb-cassette (dfrA12-orfF-aadA2). The present study indicated that Vibrio isolates from aquatic animals possessed acceptable level of sensitivity to the approved antimicrobials used in aquaculture, except moderately resistant to ampicillin.

2

Speaker(s)

Shu-Chia Hu

Topic

Lyssavirus surveillance of bat in Taiwan in 2014

Abstract

Bat is widely distributed in the world and has long lifespan. Literatures demonstrate that over 15 families of viruses can exist in bats and zoonotic pathogens, such as rabies, rabies-related lyssavirus, Nipah virus, Hendra virus and SARS-like coronavirus can transmit through bats. The surveillance of bat borne virus is getting more importance now. The genus Lyssavirus is divided into 14 species, and rabies  virus belongs to genotype 1. Lyssavirus can infect a variety of mammalian, causing rabies and rabies-like clinical signs. Animal Health Research Institute (AHRI) has conducted the bat lyssavirus survey project since 2008. A total of 119 bat brain samples of 10 species, including Pipistrellus, Pipistrellus abramus, Pipistrellus montanus, Miniopterus schreibersii, Nyctalus velutinus, Scotophilus kuhlii, Murina puta, Hipposideros armiger terasensis, Rhinolophus monoceros, Myotis sp.3, Myotis latirostris) were collected in this year, and direct fluorescent antibody test (dFA) was used to check the presence of lyssavirus antigen. All samples showed negative result. Fifteen samples of oral swabs and feces were detected negative of lyssavirus nucleic acid via RT-PCR. The collected bat sera since 2010~2014 be used to examine lyssavirus antibody through cooperation with CDC, USA in 2015.

3

Speaker(s)

Ling-Chu Hung

Topic

The capsid protein and ORF3 protein of porcine circovirus type2 were detected by specific antibodies

Abstract

Porcine circovirus 2 (PCV2) is a small, non-enveloped virus with a single-strand circular DNA genome. It is the causative agent of postweaning multisystemic wasting syndrome in pigs. Lymphocyte depletion is a hallmark of PCV2 infection. The PCV2 genome encodes for three major open reading frames (ORF), which code for the replication associated proteins (ORF1), immunogenic capsid protein (ORF2), and an apoptosis inducing protein (ORF3). The ORF3 protein of PCV2 interacts with Pirh2, an E3 ligase involved in the ubiquitination of p53, resulting in the decreased levels of Pirh2 and an increase in levels of p53, and leading to apoptosis of the virus-infected cells. The aim of this study was to provide a new profile about the lymphocyte interaction with PCV2 by detecting PCV2 associated proteins. Four 14-week old SPF (excluding PCV2) pigs were kept in one isolated room of the institute BSL3 containment facility. Blood was collected every two or three weeks. Peripheral blood mononuclear cells (PBMC) were isolated from the buffy coat fraction of blood by density centrifugation over Ficoll-Paque. PBMCs were prepared for making fluorescence assay (FA) slides and cell lysate. PCV2 capsid proteins of cell lysate were detected with the antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) by using different monoclonal antibodies (MAbs) and polyclonal antibodies (PAb). In indirect FA, MAbs (anti capsid protein or ORF3 protein) showed positive signals on those swine lymphocytes. Positive FA signals revealed by both of the MAb anti ORF3 protein and PAb anti capsid were shown on the same cellular compartments. The dual positive signals staining cells displayed small, irregular or fragmented nuclei.

4

Speaker(s)

Li-Hsuan ChenWen-Chan Li

Topic

Research and academic exchange related to international survey of avian influenza virus in wild birds in Hokkaido University, Japan.

Abstract

For the implementation of “International exchange and cooperation on bird migration and risk assessment of avian influenza/establishment of the regional partnerships for emerging zoonoses” plan, we went to Hokkaido University Veterinary Microbiology Laboratory, also the reference laboratory of avian influenza (AI), for study and research. The laboratory has good diagnostic techniques and abundant experience of research for AI, and pays close attention to activities of avian influenza in the region of Asian countries for a long time. In the process of contact, we got permission to co-participate an inoculation test of crows with the Kumamoto strain (H5N8) isolated in 2014. During the period of stay in Japan, the content of study and research included analysis and management of avian influenza virus from wild bird, learning modified neuraminidase inhibition test, and evaluation the possibility of NI test application in surveillance of serum collected from poultry farms in Taiwan. An antigenic and phylogenic analysis of H5 virus among Japan, Taiwan, and neighboring countries shows that a great antigenic variation between virus in Vietnam, neighboring isolates this year in Japan and isolates existing in Taiwan. Taiwan may not identify those new H5 viruses by H5 reference serum in time. Reference Laboratory in Japan can provide hyper-immuned serum from different antigenic H5 for emergency response of diagnosis and surveillance.