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Histochemistry Staining Techniques  

Seminar:  Histochemistry Staining Techniques



Che-Tun Hung


Ming-Hwa Jong


Histochemistry stain is an old technique. Numerous different dyes are used to characterize certain tissue constituents. It differs from the immunohistochemistry stain, which used antigen-antibody reaction to detect certain antigen in tissues. Routine histochemistry stains include hematoxylin & eosin stain, Masson’s trichrome stain, PAS stain, mucicarmine stain, alcian blue pH 2.5 stain, iron stain , acid-fast stain and toluidine blue stain, etc. Though the specificity is not high enough and these techniques are not always useful in neoplastic diseases, these techniques are still applied to several diagnostic, experimental and research fields, especially applied on certain tissue constituents. teschovirus RNA. In 2000, The Animal Health research Institute investigated a clinical case of pig farm in Nantou and the viral isolate was identified as serotype PTV. From the viruses isolated from diseased pigs in various districts of Taiwan in years 2003, 2004 and 2005, it suggests that this disease is distributed widely in Taiwan. In this study, we will perform antibodies detection from serum samples in field farms and identify diseased pigs. Based on the results of serological surveillance and virus isolation, we would know epidemiology of porcine teschovirus in Taiwan. We will design an experimental model to study the pathogenesis of porcine teschovirus. Our final goal is to develop a feasible vaccine to control the disease.