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Seminar 823  

 

Seminar:  823

1

Speaker(s)

Fan Lee

Topic

Entomological Studies at the Center for Grain and Animal Health Research, USDA

Abstract

Center for Grain and Animal Health Research, located at Manhattan, Kansas, USA, is one of the research facilities on the Northern Plains Area. The center studies arthropod-borne animal diseases, grain quality and preservation, wind erosion, genetics of hard winter wheat, and insect pests in grain and food products. This COA supporting technical visit, over a span of four months, aimed to (1) learn how to rear Culicoides sonorensis biting midges responsible for transmitting many ruminant diseases in North America, and (2) develop cell lines derived from Culicoides sonorensis tissues. Through this presentation, the method and facilities for large-scale rearing of Culicoides sonorensis at the center, and the procedures for establishing Culicoides cell cultures were introduced.

2

Speaker(s)

Jen-Chieh Chang

Topic

The Achievements of Health Monitoring Technical Platform and Study at OIE Reference Laboratory in Canada

Abstract

I The purpose of this project is to enhance the breeding stock and breeding poultry farms to improve the biosecurity and to increase their incomes through the animal health monitoring technical platform. The risk assessment of biosecurity plans, the design of management flow on farm and the disease diagnosis have been provided and applied for the breeding stock and poultry farms in 2012. In the pseudorabies (PR) elimination program igniting from 2010 to 2012, blood samples collected from 25 monitored swine breeding farms were examined for PR antigen and PR-gE antibody. Out of 25 farms, PR antigen and PR-gE antibody were not detected in 12 farms. The positive rate of PR-gE antibody was 14.11 (262/1,856) in 2012 which was decreased significantly compared to 23.64% in 2011. We also tested the PR-gE antibody in colostrums of breeding sow and the positive rate was significantly decreased from 42.95% in 2011 to 11.95% in 2012. In addition, with our devoted contribution breeder pigs and semen of a breeding farm were successfully exported to Vietnam and Palau. In goat health surveillance and CAE elimination program, we have completed testing and analysis over 446 goat serum samples. We also conducted farm-biosecurity investigation and disease consultation to 12 farms. We also held 4 discussions on technical assistance with the producer and the officers at local animal health inspection agents.

AHRI dispatched two staffs to Canadian OIE Reference Laboratory to study the rapid and confirmatory testing techniques of Chronic Waste Disease (CWD) and Bovine Spongiform Encephalopathy (BSE). CWD IHC, BioRad TeSeE® ELISA, BioRad (BSE Scrapie Discriminatory Kit) Western Blot, AMR (Advanced Meat Recovery) tests, Prionics Check-Priostrip®, Hybrid Western Blot, IDEXX Herd Check EIA, BSE IHC, necropsy, sampling technique, as well as quality assurance system and quality control were all involved. We also discussed to participate in proficiency panels from Canadian OIE Reference Laboratory and set up the tests of CWD in the future. Completion of this training greatly strengthens the diagnosis ability for BSE in Taiwan and promotes the bilateral cooperation between Taiwan and Canada.

3

Speaker(s)

Yu-Pin Liu

Topic

A Molecular Epidemiological Study of Avian Paramyxoviruses in Taiwan

Abstract

Avian paramyxovirus serotype 1 (Newcastle disease, ND) is most recognized serotype in Taiwan, however the information about the distribution of all APMVs 1-9 in domestic poultry, wild birds, imported birds and pet birds is limited. This study use molecular biology analytical techniques to determine the serotype and the distribution of APMVs in birds. Fifty-seven APMV isolates were characterized by as: 18 APMV-1, 1 APMV-2, 17 APMV-4, 8 APMV-6 and 13 APMV-7. The complete genome sequence was determined for APMV-7 strain AHRI-33, and only 36-73% identities of six amino acids with the reference APMV-7 strain in GenBank. Our results suggest the evidence for the existence of subgroups within the serotype. The results of the phylogenetic analysis of fusion protein placed 18 APMV-1 strains into genotype II of class I, genotype I, II, VI, VII of class II and one novel genotype. The present study suggests that besides ND, there are at least four more serotype of APMVs circulating in poultry and wild birds in Taiwan and this provides insight to the potential influence for avian species on epidemiology.