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The application of ELISA test in CSF antibody  

Seminar:  The application of ELISA test in CSF antibody

1

Speaker(s)

Ju- HsiaoYa

Coordinator

Shu-Hwae Lee

Abstract

Prevention with LPC vaccine and immunosurveillance for classical swine fever (CSF) are the most important issues in control and CSF eradication program in Taiwan. Serum neutralization (SN) test has been thought as the golden standard method for detection CSF, but it is not suitable for large-scale screening due to arduous and time-consuming. The experiment was aimed to compare the correlation between SN and commercial CSF antibody enzyme-linked immunosorbent assay (ELISA) kits and further evaluate the application of ELISA test on LPC vaccination program and other CSF immuno- surveillance. First, we compared the correlation between different levels of SN titers and IDEXX® CSF antibody ELISA titers. The results show that the correlation (r=0.827 ) between SN titers and IDEXX® ELISA kits,have a high r value, it is applied in following tests.We used ELISA kits to analysis serum sample from 52 farms in 2006.  The result was showed that the mean of titer was 70.68% in 827 samples (collection from the farm whose >85% positive prevalence).  This ELISA titer of marking pig vaccinated by LPC vaccine could be a reference data.  In the other experiment, the ELISA tests were performed in CSF virus contamination farms.  The results indicated that the IDEXX ELISA kit could differentiate the LPC- vaccinated  (34.67±3.18%) from CSF virus experimentally infected pigs (58.62±4.77%). According to this result, the ELISA titer was more than 47.5%, it could be suspected CFS infection pig.  These data was demonstrated that it was high correction between ELISA and SN test.  It could be perform in large scale immunosurveillance, evaluation of LPC vaccination program and efficacy, and monitor the farm whether was persistent infection by CSF virus. It can be applied in large scale of immunosurveillance, evaluation of LPC vaccination program and efficacy, and, perhaps screen of CSF virus persistent infection in pig farms.