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Seminar 822  

 

Seminar:  822

1

Speaker(s)

Kuo- Jung Tsai

Topic

The report of learning the diagnosis and surveillance techniques of chronic wasting disease in wildlife by visiting USA  The surveillance of Bovine Spongiform Encephalopathy in Taiwan

Abstract

In order to perform the international cooperation project” Studies on environmental surveillance methods and prevention strategy of important zoonoses (i.e. rabies, avian influenza, bovine spongiform encephalopathy) response to climate change”, two persons were dispatched to USA to visit the National Wildlife Research Center ( NWRC) and National Veterinary Service Laboratories ( NVSL) to learn the surveillance and prevention technique of chronic wasting disease (CWD), rabies and tuberculosis from November25 to December 9 in 2012. The diagnostic techniques and related knowledge can be applied to upgrade the operation procedures in Bovine Spongiform Encephalopathy (BSE) laboratory and also to serve as reference information for prevention and surveillance of important zoonoses in wildlife. To conduct the BSE surveillance, 8,078 brains of cattle older than 24 months of age were collected from 1998 to 2012 and subjected to enzyme-link immunosorbent assay (ELISA), western blot, histopathology, immunohistochemistry (IHC) to examine the presence of PrPSc and related lesions. In total, 7,741, 121, 11 and 205 brains were collected from cattle of routine slaughter, tuberculin positive reactors, CNS signs and other cases, respectively. The PrPSc and related lesions were not identified in all samples. The surveillance results are helpful for maintenance of controlled BSE risk. In 2012, four meetings on reviewing OIE questionnaire of BSE of Taiwan have been hosted to assist in consolidating the questionnaire and making proposal for strengthening the BSE risk control measures in Taiwan. It is also helpful to complete OIE questionnaire of BSE and to upgrade BSE risk status of Taiwan to be negligible risk. In the future, the surveillance of BSE and TSEs will be continuously conducted through learning new techniques and knowledge to prevent the invasion or spread of the disease and also to upgrade to be a negligible risk country.

2

Speaker(s)

Ming-Chung Deng

Topic

Establishment and assessment of inactived antigen purification for Foot and Mouth Disease vaccine

Abstract

In recent years, foot and mouth disease (FMD) has been spreading in Asian regions. Through to the FMD outbreak, highlight the short supply of FMD vaccines occurred in the international market. The livestock industry of Taiwan is relatively small compared to other countries that lead to difficulties for sufficient vaccine supply. Since the outbreak of FMD is often accompanied by a great loss of the agricultural industry and a severe impact on physiological fear of people, the localization of FMD vaccine manufacture is relatively a good solution for Taiwan. However, high standards of technique and high risks are required for production of FMD vaccine. Only few countries have experiences of production of FMD vaccines. Taiwan has never produced such an animal vaccine with high requirements. The project is to compare the method of FMD antigen purification between traditional PEG precipitation, sucrose gradient and ultrafiltration, and develop specificity monoclonal antibody for FMD verification of purified antigen. The sucrose gradient purified antigen can be gauged by spectrometer. The O/Taiwan/97 purified virus was also proved by specificity monoclonal antibody. Furthermore, the measure of purified antigen and specificity monoclonal antibody will apply in quality control of FMD vaccine manufacture.

3

Speaker(s)

Chun Wang

Topic

Study of porcine circovirus type 2 in Taiwan from 2000 to 2011

Abstract

Porcine circovirus type 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) in Taiwanese pig farms.  We analyzed the complete genomes of 571 PCV2 isolates in Taiwan from 2001 to 2011 and divided the isolates into two distinct genotypes, PCV2a and PCV2b.  Of the 571 Taiwanese PCV2 isolates, 22.9% (131/571) belonged to PCV2a and 77.1% (440/571) to PCV2b.  The prevalence of serum antibodies to PCV2 in pigs was investigated, and results showed that approximately 83.5% of the pigs in Taiwan were seropositive. Finishing pigs possess the highest titers of antibodies, while 9-week-old pigs contained the lowest titers for specific antibodies. Our results suggest that PCV2 infections have become common in Taiwanese pig farms.