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Seminar 847  
Date:2015-03-11
Update:2015-04-02

 

Seminar:  847

1

Speaker(s)

Chao-Fang YuYen-Lin Lee

Topic

The Report of Visiting Kaketsuken, a Japanese Animal Vaccine Factory, and Kyushu Research Station of NIAH

Abstract

To improve technical skills on large-scale production of animal vaccines and strengthen competitive ability of Taiwan, two employees were dispatched to visit Kaketsuken(The Chemo-Sero Therapeutic Research Institute), the first and the second largest animal vaccine factory for poultry and swine, in mid Dec. 2014. Through visit, discussion and enquiry, we’ve gained general knowledge offacilities, operation of large-scale production system, vaccine assays and R&D in a large vaccine factory. In addition, a senior researcher and two experienced fermentor operators from Kaketsuken have made some suggestions and resolutions to our difficulties encountered in manufacturing process for the production scale up. Besides , we’ve also visited an arthropod-borne viral disease laboratory of Kyushu Research Station of NIAH(National Institute of Animal Health, Japan) to exchange information of epidemic situation and current use of bovine ephemeral fever vaccine and its related challenge test.

2

Speaker(s)

Tien-Shine Huang

Topic

Development of Contagious Pustular Dermatitis attenuated live vaccine and its antibody detection kit

Abstract

A batch of contagious pustular dermatitis (contagious ecthyma; CE) trial subunit vaccine, 3 batches of attenuated live CE trial vaccine, and one batch of bivalent trial subunit vaccine consisting P32 of goat pox virus and F1L and B2L of CE virus recombinant proteins have been manufactured in the GMP factory of our institute. Safety, efficacy and virus contamination tests have been carried out for all these trial vaccines. After keeping in 4 for storage tests for more than 13 month, the virus titers of each CE live vaccine with stabilizer like sucrose, lactose and sorbitol, respectively showed no significant difference. Furthermore, field trials of attenuated live CE vaccine have been conducted in two private goat farms in Taichung, and the ELISA test based on F1L protein for the vaccinated animals showed that they became sero-conversion on 9th day post vaccination, and their effective titers could persist for more than 180 days. The bivalent subunit vaccine of P32, F1L and B2L recombinant proteins can provide goats protection against both pox and CE virus challenge after intramuscular injection of 20 or 60 μg per dose, and positive ELISA titers of vaccinated goat serum samples can be detected with home-made P32, B2L and F1L ELISA Kits, respectively. For adjuvant selection test for subunit vaccines, recombinant proteins were mixed and homogenized with ISA70 properly, and were injected into mice, and obvious sero-conversion happened, even with a single shot. The pregnant goats about 3 months showed no abortion and skin lesions after receiving attenuated live CE vaccine, and it means this vaccine was safely for goats. In this study, other antigenic protein genes of ORF88, ORF94 and ORF104 have been cloned into E. coli expressing plasmids. After transformation into E. coli, inducing with IPTG for expression, extraction of soluble and insoluble proteins, expression of ORF88, ORF94 and ORF104 were demonstrated in western blot with the anti-His tag antibody.

3

Speaker(s)

Chien Tu

Topic

Practical Application of Aquatic Production Medicine

Abstract

The aquatic production medicine is a medicine to prevent aquatic animals from

diseases through changes of operation procedures for production. Recently, several important aquatic animals for export have been noticed in aquaculture, including hard clam for Vietnam, bull frog for American and Japan, and oyster for Malaysia.  A series of trainings on the aquaculture and diseases prevention of clam, bull frog and oyster were held for the potential aquaculture exports in 2013.  We found that key factors affecting production are temperature, dissolved oxygen, and salinity for hard clam, feed and water body for bull frog, change of salinity for oyster.