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Seminar 860  
Date:2016-04-13
Update:2016-05-06

 

Seminar:  860  

1

Speaker(s)

Re-Shang Chen, Yu-Hua Shih

Topic

Development and Manufacture against of Biologics Duck Viral Hepatitis

Abstract

Duck viral hepatitis (DVH) characterized by hepatitis is an acute fatal disease in ducklings. The Animal Heath Research Institute used a strain of duck hepatitis A virus (DHAV) to immunize layer hens to produce eggs with high yolk antibody. All laboratory tests and field trials of this biologics development were completed in 2015. The results showed that the biologic was with a high degree of safety and efficacy to target animal. One shot of the biologic could provide protection against in duck farms. This biologic should be stored at 2 - 8 oC and its shelf life is one year. The vaccination is recommended for breeding ducks before the laying period in order to produce maternal antibody through yolk to protect ducklings from virulent DHAV infection. If the breeding ducks were immunized inappropriately, their ducklings should inject two shot on 1-day and 10-days old to receive passive immunization against virulent DHAV infection. Manufacturing license (No. 09081) has been approved by the Bureau of Animal and Plant Health Inspection and Quarantine, Taiwan, in March 2016. With a strong demand by waterfowl industry, the licence approval of this biologics would be effectively reduce economic losses caused by DHAV infection in ducks.

2

Speaker(s)

Chu-Hsiang Pan

Topic

Development of an ELISA for detection of antibodies against classical swine fever

Abstract

Classical swine fever (CSF) is a highly contagious and often fatal viral disease of pigs. To avoid outbreaks of the disease and affecting the pig industry, the LPC vaccine was used to prevention of classical swine fever in swine herds in Taiwan. Classical swine fever antibody titers can be used to assess the immune responses and vaccine efficacy; also to adjust the appropriate vaccine program by means of detecting the maternal antibody titer in piglets. Serum neutralization test (SNT) is a major method for measurement of anti-CSF antibody titer. Traditional SNT is a time-consuming and heavy labor job, including cell culture, fluorescent antibody staining and visual interpretation, which takes 3 days. Otherwise, it is a simple procedure and easy operation to detect anti-CSF antibody by ELISA. To enhance the efficiency of classical swine fever antibody detection, we cooperated with the Kaohsiung Medical University using a mammalian cell line for high level of production of glycoprotein E2 of CSF virus and constituted an anti-E2 antibody diagnostic ELISA. Compared to insect cells and yeast expression system, mammalian system is the most advanced protein expression system. It has the best post-translational modifications of proteins, retention of the best antigenicity, and improved the binding of anti-E2 antibody. To further understand the correlation between the ELISA and the SNT, we used different neutralizing antibody titers of pig sera to analyze the homemade indirect ELISA and blocking ELISA. The results showed that the r value of correlation coefficient is 0.911 and 0.877 respectively.

3

Speaker(s)

Wan-Chen Li

Topic

Genetic analysis of H10 subtype avian influenza viruses isolated from wild birds in Taiwan 2015/2016.

Abstract

According to genetic diversity, avian influenza viruses can be divided into American lineage and Eurasian lineage. Most of avian influenza viruses isolated from wild birds in Taiwan belong to Eurasian lineage. We found two intercontinental reassortant H10N7 subtype strains from wild birds surveillance in 2013. At the same time, three cases were reported that patients were infected by H10N8 and led to death in Jiangxi, China, between November 2013 and February 2014. All patients infected by H10N8 viruses had history of visiting live-poultry market. After August 2013, the prevalence of H10 subtype viruses got higher in chicken in Nanchang live poultry market. According to phylogenetic analysis, human cases were suspected to be infected by viruses of chicken origin. Although H10 subtype influenza viruses are usually low pathogenic to avian, the viruses reassortant with poultry viruses such as H9N2 may increase the risk to human. From November 2015 to March 2016, we isolated 10 strains H10 subtype viruses. We had sequenced the genomes of H10 subtype viruses since 1998, and then analyzed the sequence phylogenicity. Most viral genomes belong to Eurasian lineage. A few of viruses with mixed American lineage genes appeared occasionally, and the viruses usually existed only a season in the wild birds in Taiwan. The genome phylogenicity data can provide the clues of avian Influenza viruses evolutionary dynamic in migratory wild birds which fly through Taiwan. The six internal genes of H10 avian Influenza viruses can not only be used in H10 genetic analysis, but also be the reference gene pool for other studies.

4

Speaker(s)

Ming-Chu Cheng, Yen-Ping Chen

Topic

The Study of the Diagnosis and Surveillance of Avian Influenza in Israel

Abstract

To implement the project of ‘Establishment of Transnational Rabies and Avian Influenza Surveillance Technical Platform’, we visited Israel to learn the surveillance and the diagnosis of avian influenza and the monitoring of the bird migration routes between December 25, 2015 and January 2, 2016. We were hosted by Professor Leshem from Tel Aviv University. First, we exchanged the information of the epizootic of avian influenza (AI), the surveillance of pathogen in wildlife, and the research of bird migration with the Veterinary Services and the Israel Nature and Parks Authority. On the following, we visited Israeli Wildlife Hospital, the border of the Gaza Strip to learn about the AI problems in the poultry farms and the relationship between the wild birds and the landfills, the poultry farms suffered from AI in 2015, the Agamon Hula to understand the relationship between the cranes and the farmers, the Bio-Bee company, the bird radar at Latrun, the Jerusalem Bird Observatory and the Ein Gedi Nature Reserve. Finally, we discussed the possibility of cooperation between both countries. Israel is the endemic area of H5N1 highly pathogenic AI virus. It was believed that Taiwan’s poultry industry could benefit from learning the experience of the diagnosis and surveillance of AI from Israel. Furthermore, the researchers in Israel had a high interest in the AI surveillance and the dropping sampling in wild birds. We also shared our experience to the Israeli researchers.