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Seminar 784  
Seminar:  784
Wei-Cheng Hsu
The training report on the diagnosis techniques for emerging and re-emerging zoonoses in USA
We were dispatched to CDC in Atlanta, USA to learn the diagnosis techniques for zoonoses during September 30 to October 31 in 2009. The training topics including immunohistochemistry methods (IHC), in situ hybridization (ISH), real time RT-PCR for influenza A (H1N1), conjugate titration, direct rapid immunohistochemical test (DRIT). It is aimed to apply above diagnosis techniques to important zoonoses diagnosis and surveillance programs to assist on preventing the invasion of important zoonoses and early screening of important zoonoses. During the training course in CDC, 60 bat sera and RNA extracts of brain from Kinmen island were sent to the rabies diagnostic laboratory to examine the presence of lyssavirus antibody and lyssavirus nucleic acid. Preliminary results showed that neither lyssavirus nucleic acid nor antibody against Australian bat lyssavirus were detected. This is the first surveillance result of lyssavirus on bat in Taiwan and could be used as epidemiology information for rabies free country. We wish that the cooperation program onemerging and re-emerging zoonoses of wildlife between Taiwan and USA would be continuously maintained.
Liu Yu-Pin
Nucleotide Sequence of an Avian Paramyxovirus isolation
Avian paramyxovirus (APMV) has 9 serotypes. Among the 9 serotypes of APMV, APMV-1 (Newcastle disease virus, NDV) induces most serious poultry disease, and the other 8 serotypes APMV also cause mild disease in avian. This year, we had isolated 7 non-ND APMV strains from the samples of wild birds and imported birds. In this study, the APMV strain dove/Taipei/33/09 isolated from the pet bird was obtained from the National Taiwan University. The genome sequence was determined by the Genome Walking, 5’ and 3’ RACE (rapid amplification of cDNA ends), and PCR amplification. The PCR products were cloned into the TOPO-TA cloning vector and sequenced. Sequence compilation was carried out using the DNASTAR software package. The outcome show the virus encode six proteins, including nucleocapsid (N), phospho- (P), and matrix (M) proteins, fusion (F) and hemagglutinin-neuraminidase (HN), and large polymerase (L). The genome of the isolated APMV strain has a nucleotide sequence identity of 80% when compared with the other APMV strains in the GenBank of NCBI. These data provide the references to the APMV molecular characteristics research. Further study about the relation between sequence analysis and serology is still need, and will enable further understanding of APMV.