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Seminar 783  

Seminar:  783  
1
Speaker(s)
Shu-Hwae Lee1, Yang-Chang Tu1Jen-Chieh Chang1,
Kwok-Rong Tsai1,Wei-Cheng Hsu1, Wei-Chieh Chuang1,
Fu-Ming Wu2
Topic
A case report of aflatoxicosis in dogs
Abstract
An outbreak of unknown disease in dogs at the private animal shelter in Taipei County from late 2008 was investigated.  Affected animals showed anorexia, weight loss and dullness but some dogs died suddenly without the appearance of clinical signs.  26 dogs were submitted to autopsy in December from the shelter and the examinations of pathogenic micro-organisms, organic phosphorus and cyanide were all negative.  Surprisingly, the pathological examination revealed toxic hepatosis by the noted typical lesions. At necropsy, jaundice and hemorrhage in the subcutaneous tissue, gastrointestinal lumen filled with dark red blood were noted.  Livers were moderately to severely enlarged, icteric and had pale yellow to blackish green and rough surfaces.  Gall bladders were distended and had inspissated bile. Histopathologic findings included necrosis, degeneration and nodular regeneration of hepatocytes, perinodular fibrous tissue proliferation and biliary hyperplasia, which supported a diagnosis of toxic hepatosis.  Analysis of feed confirmed exposure to aflatoxin.  The analysis of stocked feed for aflatoxin revealed high levels of aflatoxin B1 (153 ppb, 238 ppb, 151 ppb, 192 ppb respectively).  By examinations as mentioned above, the etiology and the sudden death of toxic hepatosis was due to excessive intake of aflatoxin.  These results were similar to the scientific report published by Newman et. al. in 2007 .  After aflatoxin exposure, the affected dogs presented with liver failure, hemorrhagic diatheses and clotting abnormalities led to hemorrhage in gastrointestinal lumen and then hemorrhagic shock.  The mortality was declining gradually and markedly within 1 month since the commercial feeds containing drug prescription for hepatic protection were used.  It means dogs have powerful and effective ability of liver repairs and the prognosis of liver damage was different from human cirrhosis.  These results are worth keeping studying in the future.
2
Speaker(s)
Li-Hsuan Chen
Topic
Application of real-time Loop-mediated isothermal amplification (LAMP) for diagnosis of Q fever
Abstract
The loop-mediated isothermal amplification method uses 4 primers to recognize 6 positions on the target sequence and has the ability of amplifying a few copies of DNA to large amount of copies within an hour under isothermal condition. This reaction releasespyrophosphate from dNTP. The pyrophosphate reacts with magnesium ion causing the change of turbidity for visual identifying. By combining the simple, rapid and specific LAMP method and fluorescent metal indicator (Calcein) detection technique enable presentation of remarkably clear results. The limitation of application on lightcycler machine is 5 copies per reaction, so far. The established analysis may be helpful for diagnosis and prevention of Q fever.
3
Speaker(s)
Sue-Min Haung
Topic
Development an inactive vaccine of iridovirus for grouper
Abstract
In this project, we select the field strain 90-grouper iridovirus virus with high virulence and antigenecity as the seed virus for vaccine. The different ratio of adjuvants and administration ways were used to evaluate the efficacy of vaccine. The safety and stability test of vaccine were used to evaluate the usability for vaccine. The results show the protective indexes in vaccine mixed with adjuvants B and delivered by intraperitoneal injection is 73; the vaccine mixed with MVP adjuvants and delivered by intramuscularly injection is 50. The route of vaccine delivery by intraperitoneal injection is better than intramuscularly injection. The safety test results show no side effect using one dose or two doses vaccine to immune the fish. The stability test results show the efficacy was 70 of vaccine stored at 4 for 1 month and the efficacy was dropped to 40 of vaccine stored at 4 for 1 year. The results show the stability of the vaccine was not stable and need to improve. This year we try to improve the stability of the vaccine, to evaluate the efficacy of vaccine and to analyze the serum antibodies responses after vaccination.