Home
 /  Seminar  /  Seminar 833
Seminar 833  
Date:2014-01-08
Update:2014-08-12

Seminar:  833  

1

Speaker(s)

Chun-Hsien Tseng

Topic

Development Results of Live Attenuated and Subunit Goat Pox Vaccines

Abstract

Goat pox is a highly contagious viral disease, characterized as developing erythematous eruptions. At the preliminary stage of this disease, the goat appeared popular lesions, and followed by pustular eruptions. Possible death caused by hemorrhagic inflammation in the mucosa of  respiratory and gastrointestinal tract happened if systemic infection occurred. Goat pox virus belongs to Capripoxvirus genus of the Poxviridae family, and only one serotype exists within this virus. In the most ordinary situations, sheep pox virus (SPV) and goat pox virus (GPV) perform virus-specificity but related antigenicity exists between these two virus. GPV is about 290 x 270 nm and covered in short tubular filaments on the surface. Genome of GPV is composed of DNA in 150 Kbp.

Attenuated GPV derived from local Taiwan isolate has been experienced 72 passages in GT cells, which has as well been conducted safety study by inoculating 100 or 10 doses per goat subcutaneously into armpit area. The goat accepting 100 dose showed slight elevation in body temperature of 40.0 to 40.5 on day 5 to 7 after inoculation, but no papule lesions appeared at the inoculation sites, root of tail, or abdomen part. This result indicated R1520 experiencing 72 passages in GT performed obvious decline in virulence. The goat proved Safety-certificated of 1 dose all could be protected from wildtype virus challenge without any skin lesions. Adjuvant selection for P32 subunit vaccine has been completed in this year by immunizing Balb/c mice with the purified and re-folded P32 mixed well with adjuvants of IMS1313, ISA70, or QuilA respectively, and all mice exhibited sero-conversion based on the P32 ELISA 2 weeks after one booster, especially in the group accepting P32-ISA70 performing obvious titer evaluation after one shot. The re-folded P32 harvested from different pH value all showed similar antigenicity based on the ELISA evaluation. Goat accepting the above P32 subunit vaccine of 20 or 60μg per dose all could be protected from the wildtype virus challenge without any skin lesions, and it means the prepared P32 subunit vaccine displays highly safety and effect.

2

Speaker(s)

Tien-Shine Huang

Topic

Development of Contagious Pustular Dermatitis attenuated live vaccine and its antibody detection kit

Abstract

Goats that received a dose of trial contagious pustular dermatitis (contagious ecthyma; CE) attenuated live vaccine by intradermal inoculation developed only redness and swelling on the inoculation sites for 5-6 days post inoculation without any lesion spreading and systemic infection. Four week after vaccination, the vaccinated goats were challenged with virulent CE crust emulsion, and all the vaccinated animals were protected from the infection disease except for the mild lesions on the virulent virus inoculation sites. For the virulence reversion testthe attenuated vaccine virus has been back-passage to goats for two successive times, and each goat for the back-passages showed only mild redness and swelling on the inoculation sites of lips for 2 weeks and showed no systemic infection. In the meantime, both B2L and F1L genes of CE virus have successfully been constructed into plasmid pET24a, induced for expression, and purified, respectively. After purification, both B2L and F1L recombinant proteins were mixed and emulsified with adjuvant IMS1313 for intramuscular vaccination test of goats with each dose containing 20 or 60μg of B2L-F1L mixture subunit trial vaccine. Goats, either vaccinated with 20μg or 60μg of mixture subunit trial vaccine, their serum antibodies against B2L and F1L proteins turned to positive on 7 days post boost vaccination, and were protected from virulent virus challenge with milder lesions and 2-weeks earlier recovering of the lesions on the challenging inoculation sites comparing to the control animals. This project is aiming to develop a CE live vaccine and a highly bio-safety bivalent subunit vaccine for the prevention and control against CE epidemics in the field.

3

Speaker(s)

Shu-Hwae LEE

Topic

China annual conference on rabies and inspection report on veal production system in the Netherlands

Abstract

China 2013 annual conference on rabies was held on April 11 to 12 in Shanghai. About 280 participants including researchers of Chinese Center for Disease Control Prevention, Chinese animal epidemic prevention and control center, Chinese Academy of Agricultural Sciences, China Institute of Veterinary, Chinese Preventive Medicine Association and Agricultural Universities, and public health physicians and veterinary practicians and experts jointed. Forty-five topics including progress research on rabies, prevention strategies and control status of China, prevention and control strategies and epidemic trends of rabies in animals, the U.S. experience in rabies surveillance, rabies monitoring, characteristics bat rabies, genetic analysis of swine rabies virus, epidemic and virus characteristics of rabies in wildlife, rabies vaccine have been presented and broadly discussed. The experts acknowledge strengthening vaccination of dogs is the only means to control human rabies. A protective shield for human could only be formed when canine vaccination rate reaches 70% or higher. And the establishment of animal and human interdisciplinary multi-sectoral joint control for rabies is essential to control the spreading of rabies. Statistics show that in recent years, rabies epidemic in China Southern and eastern regions remains high also spreads to the Central Plains and northern regions. Infection with rabies in different species of livestock, such as pigs, cattle, sheep and deer etc., is gradually increasing, and lethal cases have been reported in human bitten by rabid animals.

Investigation over the Dutch veal production systems was carried out during October 15 to 25, 2013. Although 88 cases of BSE, including 84 cases of typical BSE, 4 cases of atypical BSE (2 cases of L-type and 1 case of H-type), and final one case of BSE was discovered in 2010 in cattle which was born in 2001 were reported. The Netherlands BSE risk control is believed to be international standards, which has been recognized by OIE in May 2013 and be listed in negligible BSE risk countries (BSE negligible risk country). NVWA (under the Dutch Ministry of Economic Affairs) is responsible for animal quarantine and animal food safety, and the identity and tracing back systems of the animal are well-established. In case of disease or food safety problems, the source of them may be controlled and risk control is initiated within 48 hours. It is worth to learn for us. Dutch cow membership management system is well-established, too. Very advanced processing is applied for carcasses of dead animals which is localized by satellite, animal carcasses recovery can be monitored instantly, and messages and epidemic of animal disease can then be put under control easily. Concept of animal welfare is wide accepted by Dutch people. Considerable consensus is established from producers to consumers, and there is a clear rulemaking will allow businesses to comply with. It is worth for us to follow. Currently, the Netherlands, Japan, the United States and Italy and other countries have been acknowledged as negligible BSE risk countries by OIE. Although no BSE case has been detected in Taiwan, it is listed as a BSE risk-controlled country. It is far behind many of the aforementioned BSE cases countries, and it is time to consider to up-grade the level of risk of Taiwan.

4

Speaker(s)

Yeou-Liang Lin

Topic

Comparison of the isolated porcinophilic Foot-and-Mouth Disease Virus in Taiwan

Abstract

unfortunately, Taiwan has a devastating outbreak of FMD in March 1997. Since then, the epidemics have been controlled very well; due to the massive vaccination have been performed. Taiwan gained the recognition of an FMD-free country with vaccination by the O.I.E. on May 22, 2003. In order to meet the OIE requirements for an FMD-free country without vaccination,

in total five stages have been implemented for the Staged Stopping Vaccinating Policy in pigs since April 1, 2007. However, in February 2009, outbreaks of FMD were reported from two pig farms located at Yun-Lin County and Chang-Hua County respectively. Consequently, Taiwan becomes a FMD infected country again. After that, a sporadic case was found every year. This study is to compare the VP1 sequence and antigenicity of porcinophilic FMDV isolated in Taiwan in 1998 with that in 2009 and 2012 respectively. The results shown that the similarity of VP1 sequence between 1998 isolate and 2009 isolate is 90.8%; between 1998 isolate and 2012 isolate is 89.8%. Furthermore, the r1 value of antigenic correlation between 1998 isolate and 2009 isolate is 0.52; between 1998 isolate and 2012 isolate is 0.32. It demonstrated that the variation of porcinophilic FMDV isolates has occurred year by year in Taiwan. To be the reference of making a control measure for BAPHIQ, the surveillance of viral genetic variation and viral antigenic r1 value of FMDV must been implemented.