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Seminar 861  
Date:2016-08-03
Update:2017-11-02

  

Seminar:  861  

1

Speaker(s)

Lu-Jen Ting

Topic

Invasion of exotic bovine ephemeral fever virus into Taiwan

Abstract

Bovine ephemeral fever virus is a member of the family Rhabdoviridae and bovine ephemeral fever has frequently affected cattle population in Taiwan since 1967. During the outbreaks in 2013 and 2014, exotic bovine ephemeral fever viruses were detected and nucleotide sequencing. Sequence comparison showed that the exotic viruses shared 99.0–99.4% nucleotide identities (99.4–100.0% amino acid identities) with Chinese viruses and, on the contrary, 96.2–97.2% nucleotide identities (97.8–98.6% amino acid identities) with indigenous Taiwanese viruses. Additionally, our phylogenetic analysis also supported that the newly invaded bovine ephemeral fever viruses were closely related to the Chinese strains. These exotic 2013–2014 viruses have become prevalent and displaced indigenous virus strains since their appearance.

2

Speaker(s)

Deng, Ming-Chung

Topic

The trial of FMD vaccine

Abstract

We have uesd FMD vaccine for FMD control in Taiwan. However, the major producers of FMD vaccine don’t produce the O/Taiwan/97 strain vaccine except the Russia company. According to the vaccine-matching test, the O1/Campos vaccine was selected and combined with O/Taiwan/97 vaccine for FMD control. We need to study the manufacture of FMD vaccine to response the shortage of FMD O/Taiwan/97 vaccine supply. According to the reference of FMD vaccine and the process of FMD manufacture in the major producers of FMD vaccine, we try to produce FMD vaccine in laboratory equipment. The BHK 21 cell was successfully adapted in suspension culture, the amount of cell density was 1.2 x 106/ml. The O/Taiwan/97 strain was also adapted to the suspension BHK cell, the virus titer was determined at 1.27 x 107 TCID50/ml. We use the ultrafiltration to remove the non-structure protein of FMD virus, and use the spectrophotometer to check the concentration of FMDV. The concentration of purified FMDV antigen was measured at 3.15ug/ml. We mix the oil adjuvant ISA 201 with purified FMDV antigen to make up the home-made of FMD vaccine, and immunized 8 pigs for the potency testing of neutralizing serum test. After 4 weeks of the immunization, the geometric mean of neutralizing antibody titer is 34.896, no any NSP antibody was detected at the second immunization.

3

Speaker(s)

Fan Lee

Topic

Training Seminar on the OIE PVS Tool for East Asia

Abstract

In the era of globalization, the national veterinary services play more roles in a variety of disciplines and are necessary to work in collaboration with other government agencies and interested parties. The OIE Tool for the Evaluation of Performance of Veterinary Services, or OIE PVS Tool for short, is a guide which allows countries to support veterinary services in establishing their current level of performance, identifying gaps and weaknesses in their abilities to comply with OIE international standards, and forming a shared vision with public and private interested parties. To this end, OIE Headquarters and the OIE Regional Representation for Asia and the Pacific, held the three-day training seminar at Seoul, Korea on April 26 to 28, 2016. Contents of the training included background reports of the participating delegates, introduction of OIE, preparation for PVS Evaluation, exercises, and presentation. Participants came from five delegates: China, Hong Kong, Japan, Korea, and Taiwan; the Taiwanese participants were composed of three from Bureau of Animal and Plant Inspection and Quarantine, one from Animal Disease Control Center of Changhua County, and one, Fan Lee, from the Division of Epidemiology Research of our institute.