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Seminar 818  
Date:2012-10-17
Update:2014-01-09

Seminar:  818 

1

Speaker(s)

Lu-Jen Ting

Topic

Phylogenetic analysis of goatpox viruses of Taiwanese strains and the study of PCR diagnostic techniques

Abstract

Taoyuan-2008 and Yunlin-2010 genomes exhibited 99.8% nucleotide identity over their entire length. Kenya O-240 was very similar to that of lumpy skin disease virus (LSDV), sharing 98.4% nucleotide identity but low homology to goatpox as 96.6%.  Sequence analyses of P32 gene show two main cluster: one including the Kenya O-240 vaccine strain, within the second cluster were further divided into two groups: Yemen groups, Taiwan and China and India groups. The homologies of two Taiwan Isolates, Vietnam, QL Guizhou China and S2 Guangxi isolate China were 99.7-100% identity. Using 2 primer sets targeting wild goatpox deletion region gene has been developed to differentiate diagnosis between wild type and vaccine type of goatpox virus. A total of 56 goatpox suspect goats were tested using PCR, the positive rates of wild type were 48.21% (27/56) , vaccine type were 0%; followed with nested PCR the wild type and vaccine type positive rates were 62.5 %( 35/56), 1.79 %( 1/56), respectively.

2

Speaker(s)

Fan Lee

Topic

How to Apply “Elite Program” Sponsored by Council of Agriculture: Experiences sharing

Abstract

Talent Program, sponsored by the Council of Agriculture since 2008, is a program which supports young civil servants to study overseas. The program is open to all the civil servants serving in the Council of Agriculture and the institutes which are supervised by the Council. For the applicants, age (less than 45 years old), certificate of foreign language proficiency (TOEIC scores > 750, for example), work experiences, evaluation records, and so on are basic criteria. Moreover, a research project and a ten-minute-long oral presentation are required during the administrative evaluation processing.

3

Speaker(s)

Lin Yeou-Liang

Topic

Study on the antibodies against foot-and-mouth disease virus of swine in Taiwan and 2012 report on the FAO/OIE second global conference on FMD control

Abstract

For controlling FMD effectively, the Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) addressed that a mandatory vaccination was implemented in pigs which aged greater than 12-week-old since August 2009. The effect of vaccination against FMDV is assessable via the detection of induced antibodies. To understand the distribution of antibodies against FMDV in pigs, a total of 31,075 sera collected from 2,539 farms between September 2011 and August 2012 by serum neutralization (SN) assay. The results, which were grouped and analyzed based on month, revealed that less than 29% of pig farms and 43% of pigs showed antibody negative between September 2011 and June 2012. However, the percentage of antibody negative, both of farms and pigs, decreased obviously in July and August 2012. In July 2012, less than 3% of pig farms and 12% of pigs showed antibody negative. It demonstrated that the implementation of penalty policy was effective in improving the farmers obeyed the vaccination strategies. On the other hand, according to the assessment of vaccination efficacy, a total of 2,545 sera which were collected from 309 pig farms from April to June 2012,and conducted by SN assay. This study showed that about 39% of pig farms and 51% of pigs which antibody titer were less than 1:4. It was obvious that to strengthen the percentage of vaccination against FMDV was need. Besides, the Global FMD Control Strategy was presented during an international conference organized by FAO and OIE, with the active support of the Ministry of Agriculture of Thailand. The conference was scheduled in Bangkok, Thailand, from June 26?29, 2012. More than 600 people came from 150 countries to participate the conference. The overall objective of the Global FMD Control Strategy was to contribute to poverty alleviation and improving the livelihoods in developing countries and to protect and further the global and regional trade in animals and animal products. In addition to catch the global FMD situation and the agreement of global FMD control strategy by attend to this conference, opinion exchange on FMD prevention and control strategies with foreign experts and laboratory chief was reachable. Furthermore, it was important to conduct a chance of  transboundary collaboration and introduce the concept of the Progressive Control Pathway (PCP) for global FMD control into Taiwan by attending this conference. It is crucial to improve the FMD prevention capability and to approach the final goal of elimination of FMD in Taiwan.