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Seminar 840  
Date:2014-08-13
Update:2014-09-24

Seminar:  840

1

Speaker(s)

Chen-Shen, Huang

Topic

Study of Mycobacterium bovis genotypes in 2013-2014

Abstract

Mycobacterium bovis may infect cattle and causes bovine tuberculosis (bTB) which is a chronic zoonosis. In this study, we used Spoligotyping which is a common genotyping method for Mycobacterium tuberculosis complex to analyze 39 M. bovis isolates from cattle in 2013 July to 2014 June. Three types were identified. SB0265 was the largest population (84.6%, 33/39) and distributed widely at middle and south Taiwan. The other 2 types, SB0140 and SB1040, gathered in few farms. Presently, how these farms were infected was still unknown. We need more researches with local epidemic prevention staff to clear the reasons of M. bovis infection.

2

Speaker(s)

Yang-chang, Tu

Topic

Pathological Study of Ferret-badger Rabies in Taiwan

Abstract

Taiwan was considered rabies-free and canine rabies was eliminated since 1961. The first case of ferret-badger rabies was confirmed in July, 2013. To date, 390 of 1,176 (33.2%) ferret-badgers were positive for rabies. Rabies is characterized by a nonsuppurative meningoencephalitis with intracytoplasmic Negri bodies in neurons. In this study, we found the histopathological lesions in ferret-badger rabies, and distributional trends of viral antigen were seen in 4 main brain regions: cerebrum, cerebellum, and hippocampus, and brainstem by immunohistochemistry. In total 48 cases, the lesion severity was defined by histopathological examination and 38% (18/48) showed none to slight, 31% (15/48) moderate and 31% (15/48) severe nonsuppurative meningoencephalitis. All cases showed the presence of intracytoplasmic Negri bodies in neuron. Especially, Negri bodies in hippocampus were easy to find, and then brainstem, cerebrum, and cerebellum in order. Viral antigens were most prominent in the brainstem followed by the hippocampus, cerebrum, and cerebellum. In summary, ferret-badger rabies is characterized by viral encephalitis, but the changes can be mild or even absent. Intracytoplasmic Negri bodies in neuron could be found in all cases, particularly in hippocampus. The best selective tissue for rabies virus detection in ferret-badger was the brain stem because of prominent viral antigens distribution.

3

Speaker(s)

Yu-Liang, Huang

Topic

The Outbreak of porcine epidemic diarrhea in Taiwan

Abstract

During January 20-April 30 of 2014, a total of 103 samples from 47 swine farms in 13 counties were submitted to the Animal Health Research Institute. In 20 of the 25 farms had detail histories, including severe diarrhea and vomiting affecting all ages, with approximately 100% mortality in suckling pigs. The samples were tested for transmissible gastroenteritis virus, porcine epidemic diarrhea virus (PEDV), and porcine group A rotavirus using RT-PCR. The RT-PCR of PEDV was positive in 79 samples of 34 farms. Attempts to isolate PEDV in Vero cells revealed that only 7 specimens from 7 farms showed the cytopathic effects (CPEs) of fusion and syncytia. They were confirmed as positive for PEDV by RT-PCR, sequencing, and electron microscopy. These PEDV isolates were aligned and analyzed with MEGA 5.2 software. The newly-isolated PEDV/Taiwan/2014 strains were clustered in group 2 as novel PEDV, together with strains PEDV/USA/2013, PEDV/China/2011-2013, PEDV/Korea/2014, whereas the classical CV777 strain was placed in a separate group 1. These results indicated that a novel PEDV was responsible for the recent PEDV outbreak in Taiwan.