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Seminar 794  
Date:2010-10-13
Update:2014-01-09
Seminar:  794  
1
Speaker(s)
Wang, Chun
Topic
Differential and Real-time Detection of Genotypes Porcine Circovirus Type 2 (PCV2) by Loop-Mediated Isothermal Amplification Assay (LAMP)
Abstract
Porcine circovirus type 2 (PCV2) is a 17 nm in diameter, non-enveloped virus, and contains a single–stranded circular DNA.  PCV2 can be divided into two genotypes, type 2a (PCV2a) and type 2b (PCV2b). Studies indicated that loop-mediated isothermal amplification (LAMP) is a specific, efficiency, and easy method to detect nucleic acid of various microorganisms in field samples by using two or three sets LAMP primer.  In this study, we aim to design two specific primer sets and develop a real-time LAMP system for detection and differential of PCV2a and PCV2b in field samples.  To determine the diagnostic specificity of the two genotypes LAMP assays, a total of 19 PCV2a isolates and 28 PCV2b isolates were collected and tested.  The analytical specificity for the two genotypes of PCV2 identified that only target gene could be detected, respectively, no cross-reaction were observed using two LAMP assays.  The analytical sensitivity of LAMP for the PCV2a and PCV2b were 10 copy number / reaction for each genotype of positive recombination plasmid, respectively, and the sensitivity limit of PCV2a and PCV2b These results indicated that the PCV2a and PCV2b could be detected and accurately distinguished using two genotypes of PCV2 LAMP assays with real-time monitored.  The LAMP is a specific, sensitive and quick diagnostic method for detection and differentiation of PCV2a and PCV2b.
2
Speaker(s)
Lee, Min-Shiuh
Topic
Genetic analysis of H5N2 avian influenza viruses isolated in Taiwan
Abstract
Avian influenza (AI) is a highly contagious disease caused by the type A influenza virus, which has many subtypes with respect to two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA) (16). Sixteen HA (H1–H16) and nine NA (N1–N9) subtypes have been identified (10). All of the influenza virus subtypes are found in aquatic birds, which serve as the primordial reservoir of the influenza A viruses. Among 16 HA subtypes, only H5 and H7 are highly pathogenic in poultry.
This article reports the genetic characteristics of H5N2 avian influenza viruses isolated in Taiwan between 2004 and 2010. Genetic analysis of the eight segments of the isolate indicated that these isolated virus was a reassortant whose HA and NA gene segments belonged to the American lineage and internal genes to the Eurasian lineage.
3
Speaker(s)
Tu, Chien
Topic
Survey of diseases in aquatic animals for export in 2009
Abstract
The results of survey showed that the positive rates of examined marine fish are 9% for iridovirus and 37% for nodavirus in grouper.   In freshwater fish, the positive percentage for koi herpesvirus was 9% in koi; none of viral infection was found in ornamental fish.  In penaeid shrimp, it was 64% positive for infectious hypodermal and haematopoietic necrosis virus, 9% positive for white spot disease virus, and 9% positive for Taura syndrome virus.  In abalone, none of the OIEnotifiable diseases was found.
4
Speaker(s)
Deng, Ming-Chung
Topic
Application of real time RT-PCR to identify the RNA of Sapelovirus and PEV B in swine fecal swab
Abstract
Porcine sapelovirus and enterovirus B (PEV B) are the major pathogens responsible for neurological disorders (such as encephalomyelitis), respiratory, reproductive and dermal lesions in swine. Recent studies have reported that co-infection of PEVB and other pathogenic virus such as classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV) and porcine parvovirus (PPV) usually results in severe syndromes of swine diseases in infected pigs. To investigate the infective situation of porcine sapelovirus and porcine enterovirus B in swine in Taiwan, we applied the real-time RT-PCR method to detect and differentiate the viral nucleic acid of Sapelovirus from that of PEV B. The results showed that the primers and probes exclusively designed for the detection of these two viruses could be simultaneously used in the multiplex reaction without significant interference. The sensitivity of this reaction for porcine sapelovirus and PEV B could achieve a TCID50 of approximately 102.23/ml.Therefore, the application of real-time RT-PCR can be used as the platform for rapid, accurate, and differential identification of the viral nucleic acids of Sapelovirus and PEV B. It will be more efficient and less time consuming for the virus isolation and identification of Sapelovirus and PEV B from pig fecal samples as well as for the using of conventional PCR method.