Home
 /  Seminar  /  Seminar 838
Seminar 838  

 

Seminar:  838

1

Speaker(s)

Sue-Min Huang

Topic

Genetic and virulence analysis of viral nervous necrosis virus in fishes

Abstract

Viral nervous necrosis (VNN) was first identified in 1994 and caused large economic losses in Taiwanese fish aquaculture industry during the past 20 years. In this study, the total 14 isolates were collected from various fish species in Taiwan and open reading frames encoding the coat protein (CP) gene were used for phylogenetic analysis. The nucleotide analysis of CP gene revealed that the nervous necrosis virus (NNV) isolates in Taiwan were greater than 92.7% similar to red-spotted grouper nervous necrosis virus (RGNNV) genetype, 81% similar to barfin flounder nervous necrosis virus (BFNNV) genetype, 76.6% similar to striped jack nervous necrosis virus (SJNNV) genetype and 63% similar to tiger puffer nervous necrosis virus (TPNNV) genetype. Similarly, the CP gene of NNV isolates from giant grouper (Epinephelus lanceolatus) showed high nucleotide similarity (97.2%) during 2005 to 2013. The intra-species of grouper for NNV isolates showed the nucleotide similarity were 97.5%. Among the virus propagation and viral replication efficiency test showed the higher titer at temperature of 28 °C than at 25°C.This results indicated the giant grouper nervous necrosis virus (GGNNV) was slow evolution and adapted well to environment in the field.

2

Speaker(s)

Li-Hsuan Chen

Topic

Construction of Reverse Genetic Plasmids from Avian influenza virus in 2013

Abstract

Reverse genetics of eight plasmids for the research of influenza virus is still a widely used system. Each cDNA of the eight influenza virus segments is inserted between the RNA polymerase I promoter (pol I) and terminator on pHW2000 vector. This pol I transcription unit is flanked by the RNA polymerase II promoter (pol II) and the polyadenylation signal. After transfection of eight plasmids into cell line, negative-sense vRNA and viral proteins will be synthesized by cellular pol I and pol II transcription units to process normal function of influenza virus. To realize the pathogenicity of A/CK/Penghu/1103/2012(H5N2), all 8 segments of this virus were inserted into pHW2000 vector to gain influenza virus composed of consistent gene.

3

Speaker(s)

Yu-Ju Lin

Topic

2013 Technical Cooperation Project between Taiwan & Japan-

Study of Recombinant Classical Swine Fever Virus Technology

Abstract

In January 2014, the suckling piglets exhibited severe diarrhea and death in pig farms in Pingtung and Yunlin County, Taiwan. The specimens were submitted to Animal Health Research Institute on January 21. It’s very difficult to differentiatetransmissible gastroenteritis (TGE), porcine epidemic diarrhea (PED) and porcine rotavirus infection by clinical syndromes. According to virus isolation and RT-PCR, the preliminary diagnosis was PED infection. The PED was confirmed by transmission electron microscope (TEM) examination and gene sequencing. Furthermore, the partial M gene of isolated PEDV was compared with other PEDV sequence in Genbank. The isolated PEDV was 100% identity with US strain isolated in 2013, and 98.1% identity with classical strain CV-777. Until now, the outbreak of PED has gradually slowed down. The positive rate of PEDV was 75.61% (31/41), including 31 pig farms distributed in 9 counties.