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Seminar 758  
Date:2007-10-09
Update:2014-01-09

Seminar:  758  

1

Speaker(s)

Tsu-Han Chen                       Coordinator : Hog Cholera Research Division

Topic

Development of diagnostic reagent to foot-and-mouth disease and report about studied results in the Netherlands

Abstract

For studies on diagnostic methods of foot-and-mouth disease (FMD), I went to the Central Institute for Animal Disease Control (CIDC) at Lelystad, the Netherlands. The 14-day trip was from September 11th to 24th.

The aim of the trip is to evaluate the developed diagnostic reagent to foot-and-mouth disease used by the recombinant protein. To develop non-structural protein-based in-direct ELISA for the detection of anti-NSP antibodies in swine, detection ability of the ELISA assay we developed was compared with those of the three commercially available NSP ELISA kits. Our results, based on testing 605 sera samples revealed that the specificity and sensitivity of our ELISA could be up to 100 % and 97.5 ~ 100 %, respectively. The agreement between the results of our ELISA and those of the commercial kits was high, with Kappa statistics of about 0.95.

Quantitative measurement of anti-NSP antibodies by the chromatographic strip assay for bovine and swine was also evaluated. The strips can detect antibodies to non-structure proteins of four serotypes of FMDV, the serotypes O, A, C and Asia 1, and will not react to the antibodies to swine vesicular disease virus (SVDV) and enterovirus.

The two diagnostic reagents are capable to discriminate rapidly the vaccinated animals and from the natural infected ones serologically by the laboratory.

2

Speaker(s)

Li-Hsuan Chen                      Coordinator : Hog Cholera Research Division

Topic

Study the technique of the reverse-genetics

Abstract

The reverse-genetics technique rescues live influenza virus by transfecting eight plasmids that contain eight cloned viral cDNAs into cell lines. It is very important and useful for establishing vaccine candidate in one month. Instead of using 12 plasmids in the past, combining promotor and terminator of pol I and pol II in one vector can do the replication of virus RNA and the synthesis of viral polymerase at the same time. It also decreases the number of plasmid and makes the generation of virus more efficient. 

3

Speaker(s)

Ming-Chung Deng                             Coordinator : Hog Cholera Research Division

Topic

Report for the Swine Production Immersive Knowledge Experience

Abstract

As bottleneck in the pig industry development of Taiwan, introduction of novel concepts and technologies of overseas are desperately needed for the pig industry. Thank to support of the American Soybean Association, on 24 of June it was the honor for me and my colleague Mr. Yu-Liang Lin leaving for the SPIKE held in Iowa, US. During the 4 weeks of the courses, we went to Audubon, a city placed in south and west of Iowa, with veterinary students of Iowa State University, to experience the practical operation of local pig farm and its relevant industry, obtaining further knowledge on the pig production technology and relevant key techniques. The understanding and knowledge obtained from the SPIKE is expected to bring much improvement to the prig production industry in Taiwan.