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Seminar 815  

Seminar:  815 

1

Speaker(s)

Wei-Cheng Hsu

Topic

Case report and pathological findings of swine erysipelas

Abstract

An outbreak occurred in a swine breeder farm in Yunlin County in May, 2012. The pigs developed clinical signs including anorexia, dyspnea, and high fever. Parts of the pigs died within 1-2 days after onset of clinical signs. Recovery pigs showed “diamond-skin” lesions. The mortality rate was 7% (119/1,750), 2% (8/350), and 8 % (1/12) in finishing pigs, sows, and boars, respectively. The gross lesions including cyanosis, haemorrhages in the pericardium and lung, and enlargement and congestion of the spleen were noticed. In histopathological examination, hyaline thrombi were observed in heart and lung. In microbiological examination, uniform, tiny, and α-hemolysis colonies of Erysipelothrix rhusiopathiae were cultured from liver, spleen, and kidney. The final diagnosis of swine erysipelas (SE) was confirmed by clinical signs, pathological findings, and bacterial isolation. During the last few years, SE only happened sporadically in Taiwan. However, the prevalence of SE was dramatically increasing in middle, southern, and eastern part of Taiwan since late 2011. The risk factors of SE outbreaks include vaccine discontinuation, poor herd management, and climate changing. Finally, we suggest that prevention of SE should be accomplished through biosafety control measures, animal health monitoring, and proper vaccination.

2

Speaker(s)

Jen-Huan Tsai

Topic

Research on veterinary service satisfaction and educational training model

Abstract

In order to realize the effectiveness and satisfaction of education and training in our institute, we survey four categories questionnaire in 2011, including: (1) 12  regular seminar questionnaire surveys. There are 499 copies of questionnaires and 363 returns (response rate 72.7%). The average satisfaction rate is 88.44%; (2) 12 veterinarians and farmers development education training. There are 817 copies of questionnaires and 372 returns (response rate 45.5%). The average satisfaction rate is 95.51%; (3) student dormitory satisfaction survey. There are 353 copies of questionnaires and 190 returns (response rate 53.82%). The average satisfaction rate is 89.48%; (4)  research reports and reader satisfaction surveys. There are 906 copies of questionnaires, and returned 205 (response rate 22.63%). The average satisfaction rate is 91.38%. From May to June in 2011, we sent 878 questionnaires to pig farmers in order to survey the training needs, the professional functions, farms operating difficulties and major troubling diseases. According to the compiling questionnaire results, we hold "swine production medicine and major diseases training course” twice. These courses had received very good response. In this year, we will track the training results. According to the Kirkpatrick Model, we assess the training effectiveness by four dimensions, including: (1) the response assessment; (2). learning assessment; (3). behavioral assessment; (4). the training result assessment. By means of the aforementioned training needs survey, preparing and holding the education training, and the retrospective survey of the training effectiveness for the participants, we construct a complete education and training model. We will continue to assist the industry, and train personnel to achieve sustainable industry.

3

Speaker(s)

CS Huang

Topic

Development of a formalin-inactivated nervous necrosis virus vaccine for immersion

Abstract

Nervous necrosis viruses (NNV) causing high mortality at larval stage, is one of important diseases in fishery industry. The aim of the present study was to develop a formalin-inactivated NNV vaccine for immersion and evaluate the safety and efficacy., Concentration of 0.1%0.2% and 0.3% formalin was added for inactivation at 37 for safety test. Virus titer significantly decreased after 28-hrs inactivation. The supernatant on days 3, 5 and 7 post inactivation was taken for three serial-blind passages and demonstrated no virus detected by RT-PCR. Groupers (Epinephelus malabaricus) (2 inches in length) were injected intra-peritoneally with 0.1 or 0.2 ml of formalin-inactivated vaccine. The tested groupers showed no clinical sign, side effect and no death on day14 post immunization (dpi)., Three groups of Groupers with three inches in length (aged 45 days after hatch) were immersed with formalininactivated vaccine (104, 105 and 106 TCID50/ml) for 1 hr, respectively and titer of 105.6 TCID50/ml NNV was challenged by immersion on 14, 21 and 28 dpi for efficacy test. After 21 days observation, mortality of immunized groupers was 0-11%, and 5.8-26.7% of mortality was observed in control group. The low mortality in control group might be due to loss of sensitivity in growing-up grouper. The results of our study showed no NNV was detected after formalin inactivation by RT-PCR and the vaccine of 106 TCID50/ml with 0.2% formalin provided good immune protection in Epinephelus malabaricus grouper via immersion (relative percent survival > 86.1%).